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木薯中 MAPKKK 基因家族：全基因组鉴定及干旱胁迫表达分析。

The MAPKKK gene family in cassava: Genome-wide identification and expression analysis against drought stress.

机构信息

Tropical Crops Genetic Resources Institute, Chinese Academy of Tropic Agricultural Sciences, Danzhou, Hainan, China.

College of Life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan, Hubei, China.

出版信息

Sci Rep. 2017 Nov 2;7(1):14939. doi: 10.1038/s41598-017-13988-8.

DOI:10.1038/s41598-017-13988-8
PMID:29097722
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5668296/
Abstract

Mitogen-activated protein kinase kinase kinases (MAPKKKs), an important unit of MAPK cascade, play crucial roles in plant development and response to various stresses. However, little is known concerning the MAPKKK family in the important subtropical and tropical crop cassava. In this study, 62 MAPKKK genes were identified in the cassava genome, and were classified into 3 subfamilies based on phylogenetic analysis. Most of MAPKKKs in the same subfamily shared similar gene structures and conserved motifs. The comprehensive transcriptome analysis showed that MAPKKK genes participated in tissue development and response to drought stress. Comparative expression profiles revealed that many MAPKKK genes were activated in cultivated varieties SC124 and Arg7 and the function of MeMAPKKKs in drought resistance may be different between SC124/Arg7 and W14. Expression analyses of the 7 selected MeMAPKKK genes showed that most of them were significantly upregulated by osmotic, salt and ABA treatments, whereas slightly induced by HO and cold stresses. Taken together, this study identified candidate MeMAPKKK genes for genetic improvement of abiotic stress resistance and provided new insights into MAPKKK -mediated cassava resistance to drought stress.

摘要

丝裂原活化蛋白激酶激酶激酶（MAPKKKs）是 MAPK 级联反应的一个重要单元，在植物发育和对各种胁迫的响应中发挥着关键作用。然而，关于重要的亚热带和热带作物木薯中的 MAPKKK 家族，人们知之甚少。在这项研究中，在木薯基因组中鉴定了 62 个 MAPKKK 基因，并根据系统发育分析将其分为 3 个亚家族。同一亚家族中的大多数 MAPKKK 基因具有相似的基因结构和保守基序。全面的转录组分析表明，MAPKKK 基因参与组织发育和对干旱胁迫的响应。比较表达谱显示，许多 MAPKKK 基因在栽培品种 SC124 和 Arg7 中被激活，并且 MeMAPKKKs 在 SC124/Arg7 和 W14 之间的抗旱功能可能不同。对 7 个选定的 MeMAPKKK 基因的表达分析表明，它们中的大多数在渗透、盐和 ABA 处理下显著上调，而在 HO 和冷胁迫下略有诱导。总之，本研究鉴定了候选的 MeMAPKKK 基因，可用于遗传改良非生物胁迫抗性，并为 MAPKKK 介导的木薯抗旱性提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/a225438d34f2/41598_2017_13988_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/6fc2b6b770d3/41598_2017_13988_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/da9086724dbb/41598_2017_13988_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/4ad53bf09f23/41598_2017_13988_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/ebb3edf4b313/41598_2017_13988_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/93f6aed39cab/41598_2017_13988_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/b5fc1589804a/41598_2017_13988_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/a225438d34f2/41598_2017_13988_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/6fc2b6b770d3/41598_2017_13988_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/2f9b6e8322cc/41598_2017_13988_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/da9086724dbb/41598_2017_13988_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/4ad53bf09f23/41598_2017_13988_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/ebb3edf4b313/41598_2017_13988_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/93f6aed39cab/41598_2017_13988_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/b5fc1589804a/41598_2017_13988_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb4e/5668296/a225438d34f2/41598_2017_13988_Fig8_HTML.jpg

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