Department of Biochemistry, REVA University, Bangalore, Karnataka, India.
Department of Life Sciences, Garden City University, Bangalore, Karnataka, India.
PLoS One. 2021 Nov 4;16(11):e0258657. doi: 10.1371/journal.pone.0258657. eCollection 2021.
Mitogen activated protein kinase kinase kinase (MAPKKK) form the upstream component of MAPK cascade. It is well characterized in several plants such as Arabidopsis and rice however the knowledge about MAPKKKs in tea plant is largely unknown. In the present study, MAPKKK genes of tea were obtained through a genome wide search using Arabidopsis thaliana as the reference genome. Among 59 candidate MAPKKK genes in tea, 17 genes were MEKK-like, 31 genes were Raf-like and 11 genes were ZIK- like. Additionally, phylogenetic relationships were established along with structural analysis, which includes gene structure, its location as well as conserved motifs, cis-acting regulatory elements and functional domain signatures that were systematically examined. Also, on the basis of one orthologous gene found between tea and Arabidopsis, functional interaction was carried out in C. sinensis based on an Arabidopsis association model. The expressional profiles indicated major involvement of MAPKKK genes from tea in response to various abiotic stress factors. Taken together, this study provides the targets for additional inclusive identification, functional study, and provides comprehensive knowledge for a better understanding of the MAPKKK cascade regulatory network in C. sinensis.
丝裂原活化蛋白激酶激酶激酶（MAPKKK）构成 MAPK 级联的上游成分。在拟南芥和水稻等几种植物中，它已得到很好的描述，然而，关于茶树中 MAPKKK 的知识在很大程度上尚不清楚。在本研究中，通过使用拟南芥作为参考基因组进行全基因组搜索，获得了茶树的 MAPKKK 基因。在茶树的 59 个候选 MAPKKK 基因中，有 17 个基因是 MEKK 样的，31 个基因是 Raf 样的，11 个基因是 ZIK 样的。此外，还进行了系统的结构分析，包括基因结构、位置以及保守基序、顺式作用调控元件和功能域特征，建立了茶树的系统发育关系。此外，根据在茶树和拟南芥之间发现的一个同源基因，在基于拟南芥关联模型的 C. sinensis 中进行了功能相互作用。表达谱表明，茶树的 MAPKKK 基因主要参与了对各种非生物胁迫因素的反应。综上所述，本研究为进一步全面鉴定、功能研究提供了目标，并为更好地理解 C. sinensis 中的 MAPKKK 级联调控网络提供了全面的知识。
