Huang Jiangrong, Peng Xiaochun, Zhang Kun, Li Chunyan, Su Bo, Zhang Yanxiang, Yu Wangui
Department of Intergrative Medicine, Medical School of Yangtze University, Jingzhou, Hubei 434023, P.R. China.
Department of Pathophysiology, Medical School of Yangtze University, Jingzhou, Hubei 434023, P.R. China.
Oncol Lett. 2017 Nov;14(5):5386-5392. doi: 10.3892/ol.2017.6844. Epub 2017 Aug 28.
Docking protein 2 (Dok2) and Ras p21 protein activator 1 (RASA1) are tumor suppressors which have been identified in numerous solid tumors; however, the association between their expression in breast cancer and patient prognosis remains unclear. A total of 285 consecutive patients diagnosed histopathologically with breast cancer who underwent surgery at Jingzhou Central Hospital were selected for the present study. Dok2 and RASA1 protein were explored using histopathology and western blotting techniques, and the association of patient prognosis with clinicopathological parameters was investigated using univariate and multivariate analyses. Weak expression of Dok2/RASA1 was associated with poorly differentiated breast adenocarcinomas; negatively expressed Dok2 and RASA1 were associated with increased tumor size, a higher proportion of axillary lymph node metastasis and later clinical staging. Additionally, Dok2 and RASA1 expression were associated with disease-free survival of patients with breast cancer. As indicated by Cox's regression analysis, Dok2 and RASA1 expression and the high proportion of axillary lymph node metastasis served as significant independent predictors for the recurrence of breast cancer. The results of the present study suggested that combined Dok2 and RASA1 negative expression may serve as an independent prognostic factor for patients following breast cancer surgery.
对接蛋白2（Dok2）和Ras p21蛋白激活剂1（RASA1）是在众多实体瘤中已被鉴定出的肿瘤抑制因子；然而，它们在乳腺癌中的表达与患者预后之间的关联仍不清楚。本研究选取了在荆州市中心医院接受手术的285例经组织病理学诊断为乳腺癌的连续患者。采用组织病理学和蛋白质印迹技术检测Dok2和RASA1蛋白，并通过单因素和多因素分析研究患者预后与临床病理参数的关联。Dok2/RASA1的低表达与低分化乳腺腺癌相关；Dok2和RASA1的阴性表达与肿瘤大小增加、腋窝淋巴结转移比例较高及临床分期较晚相关。此外，Dok2和RASA1的表达与乳腺癌患者的无病生存期相关。Cox回归分析表明，Dok2和RASA1的表达以及腋窝淋巴结转移比例高是乳腺癌复发的重要独立预测因素。本研究结果提示，Dok2和RASA1联合阴性表达可能是乳腺癌手术后患者的独立预后因素。
