Comprehensive analysis of DOK family genes expression, immune characteristics, and drug sensitivity in human tumors.

INTRODUCTION

is a new type of regulatory protein family that participates in the regulation of tumor cell growth. However, most of the studies are conducted in cell lines, and systematic studies have not been conducted in human tumors.

OBJECTIVES

We conducted a comprehensive analysis of based on its expression profile and its relationship with patient survival, immune infiltration, tumor microenvironment, and drug sensitivity.

METHODS

We used the TCGA database to analyze the correlation between family gene expression and prognosis and clinical stage. The protein expression of in tumor tissues was analyzed by immunohistochemistry. Use the cBioPortal database to analyze the alteration frequency in family genes in human tumors. In addition, we used ESTIMATE algorithm and TIMER website to analyze the correlation between family genes and tumor immunity. Finally, we further analyzed the relationship between family genes and tumor stemness and the sensitivity of cancer cells to chemotherapy.

RESULTS

We conducted a comprehensive analysis of family genes based on its expression profile and its relationship with patient survival. We also confirmed this conclusion by immunohistochemistry. The expression of family genes is related to OS, clinical stage, tumor mutation, methylation, CNV, and SNV. family genes are significantly associated with poor prognosis of UVM. has obvious correlation with tumor immunity. K2 can increase the sensitivity of chemotherapy drugs, while reduces the sensitivity of multiple chemotherapy drugs. In addition, the expression level of family genes is significantly correlated with the activity of cancer marker-related pathways.

CONCLUSIONS

plays a role of tumor suppressor gene or tumor-promoting gene in different tumors. However, family genes play a role in promoting cancer in UVM. family genes are significantly associated with drug sensitivity.