Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Biomed Res Int. 2017;2017:6280972. doi: 10.1155/2017/6280972. Epub 2017 Sep 20.
The root of (RAS) is a traditional Chinese medicine used for preventing and treating various diseases. In this study, we assessed RAS supplementation effects on body weight and the gene expression and methylation status in a high-fat-diet (HFD) induced obese mouse model. Female obese mice were divided into groups according to RAS dosage in diet as follows: normal diet, HFD diet (HC), HFD with low-dosage RAS (DL), HFD with medium-dosage RAS (DM), and HFD with high-dosage RAS (DH). After RAS supplementation for 4 weeks, body weight suppression and expression in DH mice were significantly higher than in HC mice, whereas no significant change in expression was detected between DM and DL mice or in their offspring. Bisulfite sequencing PCR (BSP) revealed that the CpG island in the promoter was hypermethylated up to 95.44% in the HC group, 91.67% in the DH group, and 90.00% in the normal diet group. Histological examination showed that adipocytes in the DH group were smaller than those in the HC group, indicating a potential role of RAS in obesity. This study indicated that RAS could ameliorate obesity induced by HFD and that the molecular mechanism might be associated with the expression of the gene.
(RAS)的根是一种传统的中药，用于预防和治疗各种疾病。在这项研究中，我们评估了 RAS 补充剂对高脂肪饮食 (HFD)诱导肥胖小鼠模型体重和 基因表达及甲基化状态的影响。根据饮食中 RAS 剂量，将雌性肥胖小鼠分为以下几组：正常饮食组、HFD 饮食组 (HC)、HFD 低剂量 RAS 组 (DL)、HFD 中剂量 RAS 组 (DM) 和 HFD 高剂量 RAS 组 (DH)。RAS 补充 4 周后，DH 组小鼠的体重抑制和 表达明显高于 HC 组，而 DM 和 DL 组之间或其后代的 表达无明显变化。亚硫酸氢盐测序 PCR (BSP)显示，HC 组 启动子中的 CpG 岛高度甲基化，达 95.44%，DH 组达 91.67%，正常饮食组达 90.00%。组织学检查显示，DH 组的脂肪细胞小于 HC 组，表明 RAS 可能在肥胖中发挥作用。本研究表明，RAS 可以改善 HFD 诱导的肥胖，其分子机制可能与 基因的表达有关。
