Biomedicine Research Institute and College of Life Sciences, Beijing Normal University, Beijing 100875, China.
Exp Biol Med (Maywood). 2010 Dec;235(12):1442-9. doi: 10.1258/ebm.2010.010167. Epub 2010 Oct 20.
Obesity has become a major health concern due to its strong association with the metabolic syndrome. Inhibition of adipocyte differentiation represents a key strategy to inhibit obesity. Sibiraea angustata (SA), a traditional Chinese herb, has a wide range of pharmacological effects, such as improving digestive functions. Here, we report a novel antiadipogenic effect of SA. By using the SA water extract (SAW), SA acetic ether extract (SAA) and the 3T3-L1 model of adipocyte differentiation and adipogenesis, we showed that both SAW and SAA impaired the proliferation and adipo-differentiation of 3T3-L1 in a dose- and time-dependent manner. At the molecular level, treatment of 3T3-L1 cells with SAW or SAA inhibited the expression of the key adipocyte differentiation regulator CCAAT enhancer binding protein β (C/EBPβ), as well as peroxisome proliferator activated receptor γ, adipocyte protein-2, lipoprotein lipase and glucose transporter 4. Cell cycle analysis showed that both SAW and SAA blocked cell cycle at the G1-S transition phase, causing cells to remain in the preadipocyte state. The expression of CyclinA and cyclin-dependent kinase 2 was also inhibited by SAW and SAA. Treatment with SAW also prevented the localization of C/EBPβ to the centromeres. Taken together, our results show that SA has a potent antiadipogenic effect in 3T3-L1 cells due to the inhibition of adipocyte differentiation and adipogenesis. We propose that SA may be used as a safe and effective neutraceutical to manage obesity.
肥胖症已成为一个主要的健康问题，因为它与代谢综合征密切相关。抑制脂肪细胞分化代表了抑制肥胖的一个关键策略。八角枫（SA）是一种传统的中药，具有广泛的药理作用，如改善消化功能。在这里，我们报告了 SA 的一种新的抗脂肪生成作用。通过使用 SA 水提取物（SAW）、SA 乙酸乙酯提取物（SAA）和 3T3-L1 脂肪细胞分化和脂肪生成模型，我们表明，SAW 和 SAA 均以剂量和时间依赖的方式损害 3T3-L1 的增殖和脂肪分化。在分子水平上，用 SAW 或 SAA 处理 3T3-L1 细胞抑制了关键脂肪细胞分化调节因子 CCAAT 增强子结合蛋白β（C/EBPβ）的表达，以及过氧化物酶体增殖物激活受体γ、脂肪细胞蛋白-2、脂蛋白脂肪酶和葡萄糖转运蛋白 4。细胞周期分析表明，SAW 和 SAA 均阻止细胞周期在 G1-S 过渡阶段，使细胞保持在前脂肪细胞状态。细胞周期蛋白 A 和细胞周期蛋白依赖性激酶 2 的表达也被 SAW 和 SAA 抑制。SAW 处理还阻止了 C/EBPβ向着丝粒的定位。总之，我们的结果表明，SA 通过抑制脂肪细胞分化和脂肪生成，对 3T3-L1 细胞具有强大的抗脂肪生成作用。我们提出，SA 可作为一种安全有效的营养保健品，用于治疗肥胖症。
