Ozaki-Honda Yuu, Seki Sachiko, Fujiwara Mutsunori, Matsuura Masaaki, Fujita Shuichi, Ikeda Hisazumi, Umeda Masahiro, Ayuse Takao, Ikeda Tohru
Department of Oral Pathology and Bone Metabolism, Nagasaki University Graduate School of Biomedical Sciences, 1-5-8 Sakamoto, Nagasaki, Japan.
Department of Clinical Physiology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Cancer Microenviron. 2017 Dec;10(1-3):87-94. doi: 10.1007/s12307-017-0201-1. Epub 2017 Nov 2.
Epithelial-mesenchymal transition (EMT) is a significant process in the invasion and metastasis of cancers including oral squamous cell carcinoma (OSCC), and the cadherin switch has been identified as one of the hallmarks of EMT. The aim of the present study was to evaluate the significance of the cadherin switch in the prognosis of OSCC and generate a model for prognostic predictions. Seventy-six biopsy and/or initial surgical specimens from OSCC patients were immunohistochemically analyzed for the expression of E-cadherin and N-cadherin in either overall OSCC cells in tumor nests or in OSCC cells at the invasive front. Among 76 OSCC cases, overall OSCC cells in tumor nests were negative for the expression of E-cadherin in 10 cases and positive for that of N-cadherin in 53 cases. Among 10 cases negative for the expression of E-cadherin, 4 cases were positive for that of N-cadherin. In OSCC cells at the invasive front, the expression of E-cadherin was negative in 62 cases, while that of N-cadherin was positive in 39 cases. Among 62 cases negative for the expression of E-cadherin, 33 cases were positive for that of N-cadherin. A logistic regression analysis showed that a model using the evaluation of N-cadherin expression in overall OSCC cells in tumor nests with a cut-off point of 70 years old was the best fit model. These results suggest that N-cadherin has significant value in prognostic predictions for OSCC patients.
上皮-间质转化（EMT）是包括口腔鳞状细胞癌（OSCC）在内的癌症侵袭和转移过程中的一个重要过程，钙黏蛋白转换已被确定为EMT的标志之一。本研究的目的是评估钙黏蛋白转换在OSCC预后中的意义，并建立一个预后预测模型。对76例OSCC患者的活检和/或初始手术标本进行免疫组织化学分析，以检测肿瘤巢中整体OSCC细胞或侵袭前沿的OSCC细胞中E-钙黏蛋白和N-钙黏蛋白的表达。在76例OSCC病例中，肿瘤巢中整体OSCC细胞E-钙黏蛋白表达阴性的有10例，N-钙黏蛋白表达阳性的有53例。在E-钙黏蛋白表达阴性的10例病例中，N-钙黏蛋白表达阳性的有4例。在侵袭前沿的OSCC细胞中，E-钙黏蛋白表达阴性的有62例，N-钙黏蛋白表达阳性的有39例。在E-钙黏蛋白表达阴性的62例病例中，N-钙黏蛋白表达阳性的有33例。逻辑回归分析表明，以70岁为分界点，使用评估肿瘤巢中整体OSCC细胞N-钙黏蛋白表达的模型是最佳拟合模型。这些结果表明，N-钙黏蛋白在OSCC患者的预后预测中具有重要价值。
