Misawa Kiyoshi, Imai Atsushi, Mochizuki Daiki, Misawa Yuki, Endo Shiori, Hosokawa Seiji, Ishikawa Ryuji, Mima Masato, Shinmura Kazuya, Kanazawa Takeharu, Mineta Hiroyuki
Department of Otolaryngology/Head and Neck Surgery, Hamamatsu University School of Medicine, Shizuoka, Japan.
Department of Tumour Pathology, Hamamatsu University School of Medicine, Shizuoka, Japan.
Oncotarget. 2017 Jul 18;8(44):76318-76328. doi: 10.18632/oncotarget.19356. eCollection 2017 Sep 29.
Staging and pathological grading systems are useful but imperfect predictors of recurrence in head and neck squamous cell carcinoma (HNSCC). To identify potential prognostic markers, we examined the methylation status of eight neuropeptide receptor gene promoters in 231 head and neck squamous cell carcinomas. The , , , , , , , and promoters were methylated in 80.5%, 79.2%, 67.1%, 73.2%, 35.1%, 36.4%, 38.5%, and 35.9% of the samples, respectively. In a multivariate Cox proportional hazards analysis, the odds ratio for recurrence was 2.044 (95% confidence interval [CI], 1.323-3.156; P = 0.001) when the promoter was methylated. In patients without lymph node metastasis (n = 100), methylation of (compared with methylation of the other seven genes) best correlated with poor disease-free survival (DFS) (odds ratio, 2.492; 95% CI, 1.190-5.215; P = 0.015). In patients with oral cancer (n = 69), methylated and were independent prognostic factors for poor DFS, both individually and, even more so, in combination (odds ratio, 3.90; 95% CI, 1.523-9.991; P = 0.005). Similar findings were observed for and in patients with oropharyngeal cancer (n = 162) (odds ratio, 5.663; 95% CI, 1.507-21.28; P = 0.010).
分期和病理分级系统对于预测头颈部鳞状细胞癌（HNSCC）的复发是有用的，但并不完美。为了确定潜在的预后标志物，我们检测了231例头颈部鳞状细胞癌中8种神经肽受体基因启动子的甲基化状态。其中，[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]、[此处原文缺失具体基因名称]和[此处原文缺失具体基因名称]启动子的甲基化率分别为80.5%、79.2%、67.1%、73.2%、35.1%、36.4%、38.5%和35.9%。在多变量Cox比例风险分析中，当[此处原文缺失具体基因名称]启动子甲基化时，复发的比值比为2.044（95%置信区间[CI]，1.323 - 3.156；P = 0.001）。在无淋巴结转移的患者（n = 100）中，[此处原文缺失具体基因名称]的甲基化（与其他7个基因的甲基化相比）与无病生存期（DFS）差的相关性最佳（比值比，2.492；95% CI，1.190 - 5.215；P = 0.015）。在口腔癌患者（n = 69）中，甲基化的[此处原文缺失具体基因名称]和[此处原文缺失具体基因名称]是DFS差的独立预后因素，无论是单独还是联合（比值比，3.90；95% CI，1.523 - 9.991；P = 0.005）。在口咽癌患者（n = 162）中，[此处原文缺失具体基因名称]和[此处原文缺失具体基因名称]也观察到类似结果（比值比，5.663；95% CI，1.507 - 21.28；P = 0.010）。
