• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

胰腺腺癌上调因子（PAUF）的抑制可增加胰腺癌对吉西他滨和5-氟尿嘧啶的敏感性，并抑制胰腺癌干细胞样细胞的形成。

Suppression of pancreatic adenocarcinoma upregulated factor (PAUF) increases the sensitivity of pancreatic cancer to gemcitabine and 5FU, and inhibits the formation of pancreatic cancer stem like cells.

作者信息

Cho Jae Hee, Kim Sun A, Park Soo Been, Kim Hee Man, Song Si Young

机构信息

Division of Gastroenterology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea.

Division of Gastroenterology, Department of Internal Medicine, Gachon University Gil Medical Center, Incheon, Republic of Korea.

出版信息

Oncotarget. 2017 Jul 22;8(44):76398-76407. doi: 10.18632/oncotarget.19458. eCollection 2017 Sep 29.

DOI:10.18632/oncotarget.19458
PMID:29100320
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5652714/
Abstract

Pancreatic cancer stem cells (CSCs) play a crucial role in tumorigenesis and chemoresistance of pancreatic ductal adenocarcinoma. Pancreatic adenocarcinoma up-regulated factor (PAUF), a novel secretory protein, has been shown to contribute to cancer progression and metastasis. Because the clinical relationship between PAUF and pancreatic CSCs is largely unknown, we investigated the associations between the functional role of PAUF and pancreatic CSCs. Pancreatic cancer sphere cultured from the CFPAC-1 cells showed elevated expression of PAUF and pluripotent stemness genes (Oct4, Nanog, Stat3, and Sox2), and the mRNA of PAUF were increased in CD44CD24ESA pancreatic CSCs. PAUF knockdown (shPAUF) CFPAC-1 diminished the number of spheres and decreased stemness genes and CSC surface markers (CD133, c-MET and ALDH1). In addition, siPAUF CFPAC-1 decreased the mRNA expression of multidrug resistant protein 5 (MRP5) and ribonucleotide reductase M2 (RRM2) and were more vulnerable to gemcitabine and 5-FU than negative control (p<0.05). In conclusion, PAUF was increased in pancreatic CSCs and the suppression of PAUF enhances chemotherapeutic response to gemcitabine and 5FU by decreasing MRP5 and RRM2 in pancreatic cancer cells.

摘要

胰腺癌干细胞（CSCs）在胰腺导管腺癌的肿瘤发生和化疗耐药中起着关键作用。胰腺腺癌上调因子（PAUF）是一种新型分泌蛋白，已被证明有助于癌症进展和转移。由于PAUF与胰腺CSCs之间的临床关系很大程度上未知，我们研究了PAUF的功能作用与胰腺CSCs之间的关联。从CFPAC-1细胞培养的胰腺癌球显示PAUF和多能干性基因（Oct4、Nanog、Stat3和Sox2）表达升高，并且在CD44CD24ESA胰腺CSCs中PAUF的mRNA增加。PAUF敲低（shPAUF）的CFPAC-1减少了球的数量，并降低了干性基因和CSC表面标志物（CD133、c-MET和ALDH1）。此外，siPAUF CFPAC-1降低了多药耐药蛋白5（MRP5）和核糖核苷酸还原酶M2（RRM2）的mRNA表达，并且比阴性对照更易受吉西他滨和5-氟尿嘧啶的影响（p<0.05）。总之，PAUF在胰腺CSCs中增加，抑制PAUF可通过降低胰腺癌细胞中的MRP5和RRM2增强对吉西他滨和5-氟尿嘧啶的化疗反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a0/5652714/19d86de407b5/oncotarget-08-76398-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a0/5652714/44d23aafe162/oncotarget-08-76398-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a0/5652714/96795adeab6f/oncotarget-08-76398-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a0/5652714/b3b537072af0/oncotarget-08-76398-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a0/5652714/19d86de407b5/oncotarget-08-76398-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a0/5652714/44d23aafe162/oncotarget-08-76398-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a0/5652714/96795adeab6f/oncotarget-08-76398-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a0/5652714/b3b537072af0/oncotarget-08-76398-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e1a0/5652714/19d86de407b5/oncotarget-08-76398-g004.jpg

相似文献

1
Suppression of pancreatic adenocarcinoma upregulated factor (PAUF) increases the sensitivity of pancreatic cancer to gemcitabine and 5FU, and inhibits the formation of pancreatic cancer stem like cells.胰腺腺癌上调因子（PAUF）的抑制可增加胰腺癌对吉西他滨和5-氟尿嘧啶的敏感性，并抑制胰腺癌干细胞样细胞的形成。
Oncotarget. 2017 Jul 22;8(44):76398-76407. doi: 10.18632/oncotarget.19458. eCollection 2017 Sep 29.
2
Glutathione metabolism is essential for self-renewal and chemoresistance of pancreatic cancer stem cells.谷胱甘肽代谢对于胰腺癌干细胞的自我更新和化疗耐药性至关重要。
World J Stem Cells. 2020 Nov 26;12(11):1410-1428. doi: 10.4252/wjsc.v12.i11.1410.
3
[Altered expressions of embryonic stem-related genes in pancreatic cancer stem cell].[胰腺癌干细胞中胚胎干细胞相关基因的表达改变]
Zhonghua Yi Xue Za Zhi. 2011 Nov 29;91(44):3107-10.
4
Epithelial mesenchymal transition correlates with CD24+CD44+ and CD133+ cells in pancreatic cancer.上皮间质转化与胰腺癌中的 CD24+CD44+和 CD133+细胞相关。
Oncol Rep. 2012 May;27(5):1599-605. doi: 10.3892/or.2012.1681. Epub 2012 Feb 7.
5
Expression of cancer stem cell markers in pancreatic intraepithelial neoplasias and pancreatic ductal adenocarcinomas.癌干细胞标志物在胰腺上皮内瘤变和胰腺导管腺癌中的表达。
Int J Oncol. 2012 Oct;41(4):1314-24. doi: 10.3892/ijo.2012.1565. Epub 2012 Jul 23.
6
Elevated expression of pancreatic adenocarcinoma upregulated factor (PAUF) is associated with poor prognosis and chemoresistance in epithelial ovarian cancer.胰腺腺癌上调因子 (PAUF) 的高表达与上皮性卵巢癌的不良预后和化疗耐药相关。
Sci Rep. 2018 Aug 15;8(1):12161. doi: 10.1038/s41598-018-30582-8.
7
Pancreatic adenocarcinoma upregulated factor (PAUF) confers resistance to pancreatic cancer cells against oncolytic parvovirus H-1 infection through IFNA receptor-mediated signaling.胰腺腺癌上调因子（PAUF）通过IFNA受体介导的信号传导赋予胰腺癌细胞对溶瘤性细小病毒H-1感染的抗性。
Biochem Biophys Res Commun. 2015 Apr 3;459(2):313-318. doi: 10.1016/j.bbrc.2015.02.107. Epub 2015 Feb 26.
8
Protein arginine methyltransferase 8 gene enhances the colon cancer stem cell (CSC) function by upregulating the pluripotency transcription factor.蛋白质精氨酸甲基转移酶8基因通过上调多能性转录因子来增强结肠癌干细胞（CSC）功能。
J Cancer. 2018 Apr 6;9(8):1394-1402. doi: 10.7150/jca.23835. eCollection 2018.
9
Pim-3 Regulates Stemness of Pancreatic Cancer Cells via Activating STAT3 Signaling Pathway.Pim-3通过激活STAT3信号通路调控胰腺癌细胞的干性。
J Cancer. 2017 Jun 2;8(9):1530-1541. doi: 10.7150/jca.18628. eCollection 2017.
10
Identification of stem-like cells and clinical significance of candidate stem cell markers in gastric cancer.胃癌中干细胞样细胞的鉴定及候选干细胞标志物的临床意义
Oncotarget. 2016 Mar 1;7(9):9815-31. doi: 10.18632/oncotarget.6890.

引用本文的文献

1
A six-gene prognostic signature for both adult and pediatric acute myeloid leukemia identified with machine learning.通过机器学习确定的成人和儿童急性髓系白血病的六基因预后特征。
Am J Transl Res. 2022 Sep 15;14(9):6210-6221. eCollection 2022.
2
PredictiveNetwork: predictive gene network estimation with application to gastric cancer drug response-predictive network analysis.预测网络：具有应用于胃癌药物反应预测网络分析的预测基因网络估计。
BMC Bioinformatics. 2022 Aug 16;23(1):342. doi: 10.1186/s12859-022-04871-z.
3
PAUF as a Target for Treatment of High PAUF-Expressing Ovarian Cancer.

本文引用的文献

1
Silencing pancreatic adenocarcinoma upregulated factor (PAUF) increases the sensitivity of pancreatic cancer cells to gemcitabine.沉默胰腺腺癌上调因子（PAUF）可增加胰腺癌细胞对吉西他滨的敏感性。
Tumour Biol. 2016 Jun;37(6):7555-64. doi: 10.1007/s13277-015-4641-2. Epub 2015 Dec 18.
2
Pancreatic cancer stem cells: new insight into a stubborn disease.胰腺癌干细胞：对一种难治性疾病的新见解
Cancer Lett. 2015 Feb 28;357(2):429-37. doi: 10.1016/j.canlet.2014.12.004. Epub 2014 Dec 8.
3
A PAUF-neutralizing antibody targets both carcinoma and endothelial cells to impede pancreatic tumor progression and metastasis.
PAUF作为高表达PAUF的卵巢癌的治疗靶点。
Front Pharmacol. 2022 May 6;13:890614. doi: 10.3389/fphar.2022.890614. eCollection 2022.
4
Ethanol exposure of human pancreatic normal ductal epithelial cells induces EMT phenotype and enhances pancreatic cancer development in KC (Pdx1-Cre and LSL-Kras ) mice.乙醇暴露于人胰腺正常导管上皮细胞中可诱导 EMT 表型，并增强 KC（Pdx1-Cre 和 LSL-Kras）小鼠的胰腺癌发生。
J Cell Mol Med. 2022 Jan;26(2):399-409. doi: 10.1111/jcmm.17092. Epub 2021 Dec 3.
5
GLRX3, a novel cancer stem cell-related secretory biomarker of pancreatic ductal adenocarcinoma.GLRX3，一种新型胰腺癌肿瘤干细胞相关分泌性生物标志物。
BMC Cancer. 2021 Nov 18;21(1):1241. doi: 10.1186/s12885-021-08898-y.
6
The plasticity of pancreatic cancer stem cells: implications in therapeutic resistance.胰腺癌干细胞的可塑性：对治疗耐药性的影响
Cancer Metastasis Rev. 2021 Sep;40(3):691-720. doi: 10.1007/s10555-021-09979-x. Epub 2021 Aug 28.
7
Identification of ZG16B as a prognostic biomarker in breast cancer.鉴定ZG16B作为乳腺癌的预后生物标志物。
Open Med (Wars). 2020 Nov 25;16(1):1-13. doi: 10.1515/med-2021-0004. eCollection 2021.
8
NRF2 Knockdown Resensitizes 5-Fluorouracil-Resistant Pancreatic Cancer Cells by Suppressing HO-1 and ABCG2 Expression.NRF2 敲低通过抑制 HO-1 和 ABCG2 表达使 5-氟尿嘧啶耐药的胰腺癌细胞重新敏感。
Int J Mol Sci. 2020 Jun 30;21(13):4646. doi: 10.3390/ijms21134646.
9
Markers of pancreatic cancer stem cells and their clinical and therapeutic implications.胰腺癌干细胞标志物及其临床和治疗意义。
Mol Biol Rep. 2019 Dec;46(6):6629-6645. doi: 10.1007/s11033-019-05058-1. Epub 2019 Sep 5.
10
Hallmarks of Cancer-Related Newly Prognostic Factors of Oral Squamous Cell Carcinoma.口腔鳞状细胞癌相关新预后因素的癌症标志。
Int J Mol Sci. 2018 Aug 16;19(8):2413. doi: 10.3390/ijms19082413.
一种PAUF中和抗体靶向癌细胞和内皮细胞，以阻止胰腺肿瘤的进展和转移。
Biochem Biophys Res Commun. 2014 Nov 7;454(1):144-50. doi: 10.1016/j.bbrc.2014.10.056. Epub 2014 Oct 18.
4
Efficient targeting and tumor retardation effect of pancreatic adenocarcinoma up-regulated factor (PAUF)-specific RNA replacement in pancreatic cancer mouse model.胰腺癌小鼠模型中胰腺腺癌上调因子（PAUF）特异性 RNA 替换的靶向效率和肿瘤抑制作用。
Cancer Lett. 2014 Mar 28;344(2):223-31. doi: 10.1016/j.canlet.2013.10.028. Epub 2013 Nov 1.
5
Pancreatic adenocarcinoma upregulated factor, a novel endothelial activator, promotes angiogenesis and vascular permeability.胰腺腺癌细胞上调因子，一种新型的内皮激活剂，促进血管生成和血管通透性。
Oncogene. 2013 Aug 1;32(31):3638-47. doi: 10.1038/onc.2012.366. Epub 2012 Aug 20.
6
SIRT1 inhibits proliferation of pancreatic cancer cells expressing pancreatic adenocarcinoma up-regulated factor (PAUF), a novel oncogene, by suppression of β-catenin.SIRT1 通过抑制β-catenin 抑制表达胰腺腺癌上调因子（PAUF）的胰腺癌细胞的增殖，PAUF 是一种新的癌基因。
Biochem Biophys Res Commun. 2012 Jun 29;423(2):270-5. doi: 10.1016/j.bbrc.2012.05.107. Epub 2012 May 26.
7
An analysis of human equilibrative nucleoside transporter-1, ribonucleoside reductase subunit M1, ribonucleoside reductase subunit M2, and excision repair cross-complementing gene-1 expression in patients with resected pancreas adenocarcinoma: implications for adjuvant treatment.分析接受手术切除的胰腺腺癌患者的人平衡核苷转运蛋白-1、核糖核苷酸还原酶亚基 M1、核糖核苷酸还原酶亚基 M2 和切除修复交叉互补基因-1 的表达：对辅助治疗的意义。
Cancer. 2013 Jan 15;119(2):445-53. doi: 10.1002/cncr.27619. Epub 2012 May 8.
8
An RNA aptamer that specifically binds pancreatic adenocarcinoma up-regulated factor inhibits migration and growth of pancreatic cancer cells.一种特异性结合胰腺腺癌上调因子的 RNA 适体可抑制胰腺癌细胞的迁移和生长。
Cancer Lett. 2011 Dec 26;313(1):76-83. doi: 10.1016/j.canlet.2011.08.027. Epub 2011 Sep 3.
9
c-Met is a marker of pancreatic cancer stem cells and therapeutic target.c-Met 是胰腺癌干细胞的标志物和治疗靶点。
Gastroenterology. 2011 Dec;141(6):2218-2227.e5. doi: 10.1053/j.gastro.2011.08.009. Epub 2011 Aug 22.
10
ALDH activity selectively defines an enhanced tumor-initiating cell population relative to CD133 expression in human pancreatic adenocarcinoma.ALDH 活性相对于 CD133 表达在人胰腺腺癌中选择性地定义了一个增强的肿瘤起始细胞群体。
PLoS One. 2011;6(6):e20636. doi: 10.1371/journal.pone.0020636. Epub 2011 Jun 13.