从越南稻田土壤中分离的念珠藻门蓝细菌的多样性和生物活性。
Diversity and bioactivities of nostocacean cyanobacteria isolated from paddy soil in Vietnam.
机构信息
Faculty of Biology, VNU University of Science, Hanoi, Vietnam; The Key Laboratory of Enzyme and Protein Technology, VNU University of Science, Hanoi, Vietnam.
Institute of Biotechnology, Hue University, Thua Thien Hue, Vietnam.
出版信息
Syst Appl Microbiol. 2017 Dec;40(8):470-481. doi: 10.1016/j.syapm.2017.08.001. Epub 2017 Oct 14.
Nostocacean cyanobacteria are one of the important components of paddy fields due to their ability to fix atmospheric nitrogen and supply phytohormones for crop growth. In this study, 13 Nostoc strains isolated from paddy soils in Vietnam were classified using a polyphasic approach. The results showed a high diversity of the isolated strains that represented seven morphotypes corresponding to five genotypes, with 16S rRNA gene sequence similarity values ranging between 94.97-99.78% compared to the available sequences from GenBank. Bioassay assessment revealed that 11 out of 13 strains possessed antibacterial activities, three of which exhibited cytotoxic activities on MCF7 and HCT116 cells with an IC ranging from 47.8μgmL to 232.0μgmL. Interestingly, strains with identical 16S rRNA gene sequences displayed different antibacterial and cytotoxic activity profiles.
念珠藻蓝细菌是稻田的重要组成部分之一，因为它们能够固定大气中的氮并为作物生长提供植物激素。在这项研究中，从越南稻田土壤中分离出的 13 株念珠藻采用多相分类法进行分类。结果表明，分离株具有很高的多样性，代表了七种形态型，对应于五个基因型，与 GenBank 中可用序列的 16S rRNA 基因序列相似性值在 94.97％-99.78％之间。生物测定评估表明，13 株中的 11 株具有抗菌活性，其中 3 株对 MCF7 和 HCT116 细胞具有细胞毒性活性，IC 范围为 47.8μgmL 至 232.0μgmL。有趣的是，具有相同 16S rRNA 基因序列的菌株表现出不同的抗菌和细胞毒性活性谱。
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