Arabidopsis Novel Glycine-Rich Plasma Membrane PSS1 Protein Enhances Disease Resistance in Transgenic Soybean Plants.

Nonhost resistance is defined as the immunity of a plant species to all nonadapted pathogen species. Arabidopsis () ecotype Columbia-0 is nonhost to the oomycete plant pathogen and the fungal plant pathogen that are pathogenic to soybean (). Previously, we reported generating the mutation in the genetic background as well as genetic mapping and characterization of the Arabidopsis nonhost resistance -susceptible gene locus, In this study, we identified six candidate genes by comparing single-nucleotide polymorphisms of (1) the bulked DNA sample of seven F families homozygous for the allele and (2) the mutant with Columbia-0. Analyses of T-DNA insertion mutants for each of these candidate genes identified the gene encoding a glycine-rich protein as the putative gene. Later, complementation analysis confirmed the identity of as the gene. is induced following infection as well as expressed in an organ-specific manner. Coexpression analysis of the available transcriptomic data followed by reverse transcriptase-polymerase chain reaction suggested that is coregulated with (), a core gene in autophagy. PSS1 contains a predicted single membrane-spanning domain. Subcellular localization study indicated that it is an integral plasma membrane protein. Sequence analysis suggested that soybean is unlikely to contain a PSS1-like defense function. Following the introduction of into the soybean cultivar Williams 82, the transgenic plants exhibited enhanced resistance to , the pathogen that causes sudden death syndrome.