Parween Daraksha, Sahu Binod Bihari
Laboratory of Molecular Genetics and Plant Immunity, Department of Life Science, National Institute of Technology Rourkela, Odisha 769008 India.
Curr Res Microb Sci. 2022 Feb 8;3:100109. doi: 10.1016/j.crmicr.2022.100109. eCollection 2022.
There is neither resistant rice cultivar nor any control measure against AG-1 IA (), causal of sheath blight and a major threat to global rice production. Rice is a host and Arabidopsis is a nonhost with underlying nonhost resistance (NHR) gene which is largely untested. Using approaches of forward genetics and tools, cytology, and molecular biology, we identified homozygous mutants in Arabidopsis, mapped the NHR gene, and functionally characterized it in response to was mapped on Ch 4 between and (844.6 Kb) and identified as the candidate gene. We found that breach of immunity in by activates defense responses whereas photosynthetic pigment biosynthesis and developmental processes are negatively regulated. In addition, a gradual decrease in by 3 dpi revealed that positively regulated early SA-mediated resistance. Whereas increased expression of by 3 dpi supported switching to necrotrophy, SA-mediated defense in Col-0 leading to immune response. Enhanced expression of in rss1 supported autophagic cell death. , and function together to provide NHR against . These findings demonstrate that provides NHR against in Col-0 that evoke SA-mediated early immunity with boulevard for potential biotechnological application.
目前既没有抗纹枯病的水稻品种，也没有针对引起纹枯病的AG-1 IA（）的防治措施，AG-1 IA对全球水稻生产构成重大威胁。水稻是宿主，拟南芥是非宿主，具有潜在的非宿主抗性（NHR）基因，但该基因在很大程度上未经测试。我们采用正向遗传学方法以及细胞学和分子生物学工具，在拟南芥中鉴定出纯合突变体，定位了NHR基因，并对其在响应时的功能进行了表征。该基因定位于第4号染色体上和之间（844.6 Kb），并鉴定为候选基因。我们发现，通过激活防御反应而突破中的免疫，而光合色素生物合成和发育过程受到负调控。此外，在3 dpi时逐渐下降表明正向调控早期SA介导的抗性。而在3 dpi时的表达增加支持转向坏死营养型，在Col-0中SA介导的防御导致免疫反应。rss1中表达的增强支持自噬细胞死亡。、和共同发挥作用，提供针对的NHR。这些发现表明，在Col-0中提供针对的NHR，引发SA介导的早期免疫，为潜在的生物技术应用提供了途径。
