Rathnakar Bharath, Rao Bola Sadashiva Satish, Prabhu Vijendra, Chandra Subhash, Mahato Krishna Kishore
Department of Biophysics, School of Life Sciences, Manipal University, Manipal, Karnataka, 576104, India.
Department of Radiation Biology and Toxicology, School of Life Sciences, Manipal University, Manipal, Karnataka, 576104, India.
Lasers Med Sci. 2018 May;33(4):699-707. doi: 10.1007/s10103-017-2371-y. Epub 2017 Nov 3.
Management of burn injuries are a growing concern, especially in determining the progression of healing. Several techniques are being practiced in clinics and have been considered all-time standard approaches to determine pre- and post-treatment outcomes of a healthy healing. However, these kinds of methods involve repeated biopsies and thereby hindering tissue repair. In view of this, our perspective was to develop a non-invasive tool in an attempt to provide a solution to determine the progression of healing, in vivo. Hence, the present study was designed to investigate the ability of laser-induced fluorescence (LIF) to monitor the variations in collagen intensity at various time points (0, 2, 6, 12, 18, 24, and 30 days) during burn tissue repair in mice, post low-power laser therapy (LPLT). The spectral findings demonstrated a significant change in collagen intensity as observed on day 24 (p < 0.05) and 30 (p < 0.01), when treated with LPLT (830 nm 3 J/cm) as compared to untreated control. From the observation, it was evident that the LIF could objectively monitor the progression of burn tissue repair in vivo.
烧伤的管理日益受到关注，尤其是在确定愈合进程方面。临床上正在采用多种技术，并且这些技术一直被视为确定健康愈合的治疗前和治疗后结果的标准方法。然而，这类方法需要反复进行活检，从而阻碍组织修复。有鉴于此，我们的想法是开发一种非侵入性工具，试图提供一种在体内确定愈合进程的解决方案。因此，本研究旨在调查激光诱导荧光（LIF）监测小鼠烧伤组织修复过程中不同时间点（0、2、6、12、18、24和30天）胶原强度变化的能力，这些时间点是在低功率激光治疗（LPLT）之后。光谱结果表明，与未治疗的对照组相比，在接受LPLT（830 nm，3 J/cm）治疗后，在第24天（p < 0.05）和第30天（p < 0.01）观察到胶原强度有显著变化。从观察结果来看，很明显LIF可以在体内客观地监测烧伤组织的修复进程。
