Department of Biophysics, School of Life Sciences, Manipal Academy of Higher Education, Manipal, India.
Department of Biotechnology, School of Life Sciences, Manipal Academy of Higher Education, Manipal, India.
J Biophotonics. 2018 Sep;11(9):e201700394. doi: 10.1002/jbio.201700394. Epub 2018 May 16.
The focus of the current study was to interrogate the predictive potential of laser-induced autofluorescence (LIAF) by objectively assessing collagen synthesis in burn wound granulation tissues ex vivo. Prior grafting, granulation tissues (20 samples) following burn injury were collected from 17 subjects of age range 18 to 60 years with patient/donor consent and the corresponding autofluorescence spectra were recorded at 325 nm He-Cd laser (≈2 mW) excitations. The resulting endogenous collagen intensity from the above tissue samples was computed by normalizing the nicotinamide adenine dinucleotide levels. In addition, the hydroxyproline content was also estimated biochemically from the same granulation tissues. A comparative assessment of both LIAF and biochemical estimations for endogenous collagen by hydroxyproline resulted in strong positive correlation among them. The above relevant observations suggest that LIAF is equally informative as that of biochemical estimations, in evaluating endogenous collagen content in wound granulation tissues. Thus, it can be concluded that LIAF has the predictive potential, as a noninvasive objective tool to measure the endogenous collagen levels in wound biopsy tissues and provide complementary data conducive for making clinical decisions.
本研究的重点是通过客观评估烧伤创面肉芽组织中的胶原合成，探讨激光诱导自发荧光（LIAF）的预测潜力。在进行植皮之前，从年龄在 18 至 60 岁之间的 17 名患者/供体中收集烧伤后产生的肉芽组织（20 个样本），并在 325nm 氦镉激光（≈2mW）激发下记录相应的自发荧光光谱。通过对烟酰胺腺嘌呤二核苷酸水平进行归一化处理，计算出上述组织样本中内源性胶原的强度。此外，还从相同的肉芽组织中通过生物化学方法估算羟脯氨酸含量。LIAF 和羟脯氨酸的内源性胶原生化评估的比较评估表明，它们之间存在很强的正相关性。上述相关观察结果表明，LIAF 与生化评估同样具有信息量，可用于评估创面肉芽组织中的内源性胶原含量。因此，可以得出结论，LIAF 具有预测潜力，可作为一种非侵入性的客观工具，用于测量创面活检组织中的内源性胶原水平，并提供有助于做出临床决策的补充数据。
