State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; Beijing University of Chemical Technology, Beijing 100029, China.
Beijing University of Chemical Technology, Beijing 100029, China.
Bioresour Technol. 2018 Feb;249:751-757. doi: 10.1016/j.biortech.2017.08.068. Epub 2017 Aug 16.
As the most representative functional sugar, the application areas and market demands of xylo-oligosaccharides (XOS) have been expanding year by year. Owing to the complex structure of corn stalk (CS), XOS obtained from CS are accompanied by problems such as low purity and high color value, which degrade the product. To improve the quality of XOS from CS, the enzymatic hydrolysis was precipitated by acid; then, the ethanol elution concentration was systematically investigated after optimizing the adsorption conditions. The results showed that the purity of XOS was increased to 87.28% from 67.31%, and the color value was decreased to 1050 from 4682 when the acid precipitation pH was 2. On the basis of acid precipitation, if the corresponding optimal conditions of XOS adsorption and elution were used, the highest purity of XOS was 97.87% obtained, with the lowest color value, 780, which reached the standard of the commercial XOS.
作为最具代表性的功能性糖，木低聚糖（XOS）的应用领域和市场需求逐年扩大。由于玉米秸秆（CS）结构复杂，从 CS 中获得的 XOS 存在纯度低、色值高等问题，降低了产品质量。为提高 CS 中 XOS 的质量，采用酸沉淀对酶解产物进行预处理；然后，在优化吸附条件后，系统研究了乙醇洗脱浓度。结果表明，当酸沉淀 pH 值为 2 时，XOS 的纯度从 67.31%提高到 87.28%，色值从 4682 降低到 1050。在酸沉淀的基础上，如果采用相应的 XOS 吸附和洗脱最佳条件，可获得最高纯度为 97.87%的 XOS，色值最低为 780，达到了商品 XOS 的标准。
