State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China; State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Bioresour Technol. 2018 Feb;249:342-347. doi: 10.1016/j.biortech.2017.09.203. Epub 2017 Oct 4.
As the most representative of lignocellulosic materials, corn stalk (CS) will be a great candidate to produce xylo-oligosaccharides (XOS). Owing to the high impurity content of the XOS produced by directly enzymatic hydrolysis of xylan extracted from CS, subsequent refining steps are essential. The present study was aimed to investigate desorption during ethanol elution to improve the quality and antioxidant activity of XOS from CS. The desorption was systematically investigated after optimizing the elution conditions. The results showed that it had an elution watershed when the volume ratio was 2:1. More interestingly, XOS had a obvious priorities of desorption during ethanol gradient elution. The highest purity of XOS was 98.12% from 30% ethanol eluate. Antioxidant activity assay showed that the highest radical scavenging activity of XOS was 89.89% obtained from 70% ethanol eluate at a concentration of 3 mg/mL, which could be used in antioxidant food, feed additives.
作为最具代表性的木质纤维素材料，玉米秸秆（CS）将是生产木二糖（XOS）的绝佳候选材料。由于直接从 CS 中提取的木聚糖经酶解产生的 XOS 杂质含量较高，因此后续的精制步骤必不可少。本研究旨在探讨乙醇洗脱过程中的解吸作用，以提高 CS 来源 XOS 的质量和抗氧化活性。在优化洗脱条件后，对解吸作用进行了系统研究。结果表明，当体积比为 2:1 时，出现洗脱分水岭。更有趣的是，在乙醇梯度洗脱过程中，XOS 具有明显的优先解吸顺序。从 30%乙醇洗脱液中可获得纯度最高为 98.12%的 XOS。抗氧化活性测定表明，在 3mg/mL 浓度下，从 70%乙醇洗脱液中得到的 XOS 的自由基清除活性最高，为 89.89%，可用于抗氧化食品、饲料添加剂。
