Zaninovic Nikica, Irani Mohamad, Meseguer Marcos
The Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, New York, New York.
Instituto Valenciano de Infertilidad, Universidad de Valencia, Valencia, Spain.
Fertil Steril. 2017 Nov;108(5):722-729. doi: 10.1016/j.fertnstert.2017.10.002.
Time-lapse microscopy (TLM) is an exciting novel technology with great potential for enhancing embryo selection in the embryology laboratory. This non-invasive objective assessment of embryos has provided a new tool for predicting embryo development and implantation potential. TLM detects several morphological phenomena that are often missed with static observations using conventional incubators, such as irregular divisions, blastocyst collapse and re-expansion, timing of blastocoel appearance, and timing of formation and internalization of fragments. Nevertheless, it should be recognized that conventional morphological assessment has been widely accepted as the gold standard by most embryologists. TLM can enhance conventional morphological assessments to improve embryo selection and subsequent reproductive outcomes. Furthermore, morphokinetic parameters can aid in differentiating between euploid and aneuploid embryos, although they are not sufficiently accurate to replace preimplantation genetic testing for aneuploidy. Morphokinetic assessment together with chromosomal screening may ultimately help identify euploid embryos with the highest developmental potential.
延时显微镜技术（TLM）是一项令人兴奋的新技术，在胚胎学实验室中具有极大潜力，可用于改进胚胎选择。这种对胚胎的非侵入性客观评估为预测胚胎发育和着床潜力提供了一种新工具。TLM能检测到一些使用传统培养箱进行静态观察时常常遗漏的形态学现象，如不规则分裂、囊胚塌陷与再扩张、囊胚腔出现的时间以及碎片形成和内化的时间。然而，应当认识到，传统形态学评估已被大多数胚胎学家广泛接受为金标准。TLM可强化传统形态学评估，以改善胚胎选择及后续的生殖结局。此外，尽管形态动力学参数在区分整倍体和非整倍体胚胎方面不够准确，尚不足以取代非整倍体的植入前基因检测，但它能辅助进行这种区分。形态动力学评估与染色体筛查相结合最终可能有助于识别出发育潜力最高的整倍体胚胎。
