使用延时明场和光学相干显微镜对小鼠胚胎质量进行无标记评估。

Label-free evaluation of mouse embryo quality using time-lapse bright field and optical coherence microscopy.

作者信息

Wang Fei, Hao Senyue, Park Kibeom, Ahmady Ali, Zhou Chao

机构信息

Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO, USA.

Department of Electrical & Systems Engineering, Washington University in St. Louis, Saint Louis, MO, USA.

出版信息

Commun Biol. 2025 Apr 15;8(1):612. doi: 10.1038/s42003-025-08044-5.

DOI:10.1038/s42003-025-08044-5

PMID:40234728

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12000469/

Abstract

The selection of high-quality embryos is essential to enhance the implantation rate for in vitro fertilization (IVF). Optical coherence microscopy (OCM) can noninvasively provide three-dimensional (3D) high-resolution imaging of developing embryos. The revealed microstructures can be used for accurate embryo evaluation. Here, we acquire time-lapse 3D OCM images with co-registered bright-field imaging on mouse embryo development from the one-cell stage to the fully hatched blastocyst inside an incubator. Our results demonstrate the capability of OCM to detect structural features of the developing embryos. The second and third embryonic cell cycles are indicated to be associated with blastocyst formation and the hatching capability. OCM-based time-lapse technology holds the potential to enrich early embryo development insights and streamline embryo selection within IVF clinics.

摘要

选择高质量胚胎对于提高体外受精（IVF）的着床率至关重要。光学相干显微镜（OCM）可以无创地提供发育中胚胎的三维（3D）高分辨率成像。所揭示的微观结构可用于准确的胚胎评估。在此，我们在培养箱内对小鼠胚胎从单细胞阶段到完全孵化的囊胚的发育过程进行了具有共配准明场成像的延时3D OCM图像采集。我们的结果证明了OCM检测发育中胚胎结构特征的能力。第二和第三个胚胎细胞周期被表明与囊胚形成和孵化能力有关。基于OCM的延时技术有潜力丰富对早期胚胎发育的认识，并简化IVF诊所内的胚胎选择。