Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh, India.
Department of Environment Studies, Panjab University, Chandigarh, India.
Int J Pharm. 2018 Jan 15;535(1-2):95-105. doi: 10.1016/j.ijpharm.2017.10.059. Epub 2017 Nov 2.
In the present study, we have synthesized mixed cuprous/copper oxide nanosuspensions by metallosurfactant based microemulsion technique. Three metallosurfactants were synthesized which includes two non-ionic double chained metallosurfactants with C, C chains with coordinated copper i.e. Cudda and Cuhexa, respectively. Another cationic double chained metallosurfactant with loosely bound metal (Cuctac) was also prepared. The prepared metallocomplexes were characterized using FTIR, elemental analysis, and NMR. The effect of the position of metallosurfactant in microemulsion on the fabrication and properties of nanosuspensions was elucidated. In this method, no external reducing agent and capping agent were added and tween 80 acted both as reducing and stabilizing agent for the nanoparticles. The synthesized nanoparticles were characterized and it was observed that mixed copper and cuprous oxide particles are present in colloidal suspension for all the three studied metallosurfactants. The kinetics of formation of mixed copper/cuprous oxide nanosuspensions (Ns) and their stability was estimated using Uv-visible spectroscopy. Further, the binding and interactions of copper nanosuspensions with calf Thymus DNA (CT-DNA) were assessed using Uv-vis spectroscopy, circular dichroism and gel electrophoresis. Additionally, the antioxidant activity of the Cu Ns was checked using DPPH assay. The role of positive charge on nanoparticles as evaluated from Zeta potential was responsible for DNA affinity. The DNA conformational changes in the presence of nanosuspensions and relevant scavengers were investigated. Further, the anti-proliferative activity of copper Ns was assessed using HeLa cells and Cuhexa derived Ns were proved to be active with highest activity at a low concentration and were nontoxic towards normal cell lines. In summary, this work demonstrates a softer approach for the synthesis of copper nanosuspensions with a size range of 2-5 nm and evaluated the role of type and structure of metallosurfactant on size, stability of particles and anti-proliferative activity.
在本研究中，我们通过基于金属表面活性剂的微乳液技术合成了混合的氧化亚铜/氧化铜纳米悬浮液。我们合成了三种金属表面活性剂，其中包括两种带有配位铜的非离子型双链金属表面活性剂，即 Cudda 和 Cuhexa。另一种带有松散结合金属的阳离子型双链金属表面活性剂（Cuctac）也被制备出来。所制备的金属配合物使用傅里叶变换红外光谱（FTIR）、元素分析和核磁共振（NMR）进行了表征。阐明了微乳液中金属表面活性剂的位置对纳米悬浮液的制备和性质的影响。在这种方法中，没有添加外部还原剂和封端剂，而吐温 80 既充当了纳米粒子的还原剂，也充当了稳定剂。合成的纳米粒子进行了表征，结果表明，对于所有三种研究的金属表面活性剂，混合的铜和氧化亚铜颗粒都存在于胶体悬浮液中。使用紫外可见光谱法估算了混合铜/氧化亚铜纳米悬浮液（Ns）的形成动力学及其稳定性。此外，使用紫外可见光谱、圆二色性和凝胶电泳评估了铜纳米悬浮液与小牛胸腺 DNA（CT-DNA）的结合和相互作用。此外，使用 DPPH 测定法检查了 Cu Ns 的抗氧化活性。从 Zeta 电位评估的纳米粒子的正电荷的作用负责 DNA 亲和力。研究了纳米悬浮液和相关清除剂存在时 DNA 构象的变化。此外，使用 HeLa 细胞评估了铜纳米悬浮液的抗增殖活性，并且证明 Cuhexa 衍生的纳米悬浮液具有活性，在低浓度下具有最高的活性，并且对正常细胞系没有毒性。总之，这项工作展示了一种更温和的方法来合成尺寸范围为 2-5nm 的铜纳米悬浮液，并评估了金属表面活性剂的类型和结构对粒径、颗粒稳定性和抗增殖活性的作用。
