a Department of Chemistry and Centre of Advanced Studies in Chemistry , Panjab University , Chandigarh , India.
b Department of Environment Studies , Panjab University , Chandigarh , India.
J Biomol Struct Dyn. 2019 Mar;37(4):892-909. doi: 10.1080/07391102.2018.1442251. Epub 2018 Feb 27.
The present work aims at the fabrication of iron oxide nanocolloids using biocompatible microemulsion and their cytotoxic, genotoxic effect on Vitis vinifera plant has been evaluated. The three iron-based metallosurfactant complexes were synthesized. Nanosuspensions (Ns) were prepared using microemulsion technique and for the purpose, the microemulsion was prepared using oleic acid, butanol, tween 80 and as synthesized iron metallosurfactant. In this technique, no additional capping agent and/or reducing agent was added. Tween 80 which is a biocompatible surfactant acted as a reducing agent as well as stabilizing for the iron oxide Ns. Characterization of Ns's was done using TEM, FESEM, EDX, XRD, AFM, and zeta potential. Mixed type of iron oxide nanoparticles i.e. magnetite (FeO), and maghemite (FeO) with a size range of 1-16 nm was found to be present in the nanosuspensions prepared from all the three precursors. The antioxidant activity of the Fe Ns was also confirmed using DPPH assay, with order of activity FeDDA > FeCTAC > FeHEXA. The cellular toxicity of Ns was evaluated by observing the morphological changes on V. vinifera plant (petiole) using a light microscope. Further, the interactions of iron oxide Ns with V. vinifera's DNA (plant-DNA) was assessed using circular dichroism (CD) and gel electrophoresis. For the case of FeCTAC Ns, a decrease in the intensity of bands was observed indicating fragmentation or adduct formation resulting in DNA damage. In the case of FeDDA, a modest decrease in the intensity of bands was observed. However, for FeHEXA Ns, complete neutralization of bands was confirmed implying maximum damage to the plant DNA. CD, gel electrophoresis and antioxidant activity confirmed that FeHEXA Ns were most toxic and FeDDA Ns were safest among the three as-fabricated nanosuspensions.
本工作旨在使用生物相容性微乳液制备氧化铁纳米胶体，并评估其对酿酒葡萄植物的细胞毒性和遗传毒性。合成了三种铁基金属表面活性剂配合物。使用微乳液技术制备纳米混悬剂（Ns），为此，使用油酸、丁醇、吐温 80 和合成的铁金属表面活性剂制备微乳液。在该技术中，没有添加额外的封端剂和/或还原剂。吐温 80 是一种生物相容性表面活性剂，既作为还原剂，又作为氧化铁 Ns 的稳定剂。使用 TEM、FESEM、EDX、XRD、AFM 和 ζ 电位对 Ns 进行了表征。从所有三种前体制备的纳米混悬剂中发现存在混合类型的氧化铁纳米颗粒，即磁铁矿（FeO）和磁赤铁矿（FeO），粒径范围为 1-16nm。使用 DPPH 测定法还证实了 Fe Ns 的抗氧化活性，活性顺序为 FeDDA > FeCTAC > FeHEXA。通过使用光显微镜观察酿酒葡萄植物（叶柄）的形态变化来评估 Ns 的细胞毒性。进一步，使用圆二色性（CD）和凝胶电泳评估氧化铁 Ns 与酿酒葡萄的 DNA（植物-DNA）的相互作用。对于 FeCTAC Ns 的情况，观察到带的强度降低，表明 DNA 发生片段化或加合物形成，导致 DNA 损伤。对于 FeDDA 的情况，观察到带的强度略有降低。然而，对于 FeHEXA Ns，证实了带的完全中和，这意味着对植物 DNA 的最大损伤。CD、凝胶电泳和抗氧化活性证实，FeHEXA Ns 是三种制备的纳米混悬剂中最毒的，FeDDA Ns 是最安全的。
