Department of Environment Studies, Panjab University, Chandigarh, India.
Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh, India.
Colloids Surf B Biointerfaces. 2018 Oct 1;170:99-108. doi: 10.1016/j.colsurfb.2018.05.069. Epub 2018 May 30.
In the present study, we have synthesized nickel hydroxide nanosuspensions (Ns) using microemulsion technique. This approach is eco-friendly and makes use of Tween 80 (a non-ionic biocompatible surfactant) and newly synthesized metallosurfactants for the formation of uniform nanoparticles in the form of nanosuspensions (Ns). The nickel hydroxide Ns's were derived from three different metallosurfactants i.e. NiCTAC (Bishexadecyltrimethylammonium nickel tetrachloride), NiDDA (Bisdodecylamine nickel dichloride) and NiHEXA (bishexadecylamine nickel dichloride). Three different nickel-based metallosurfactants were synthesized and characterized using various methods such as CHN, HNMR, and FTIR. Fabrication of nanosuspension was confirmed using different characterization methods such as Transmission electron microscopy (TEM), Field Emission Scanning Electron Microscope (FESEM), Energy Dispersive X-ray Spectroscopy (EDX), X-ray Diffraction (XRD), pH and Zeta potential. These particles were further investigated for their genotoxic and cytotoxic effects on gram-negative bacteria, Escherichia coli (E. coli). Effect of nanosuspensions on E. coli was confirmed using colony forming unit count, agar well diffusion, and gram staining method. Through colony forming unit count method, nanosuspensions influence on the colony-forming capacity of E. coli cells was confirmed. Agar well diffusion method provides the estimation of antimicrobial activity, and the largest inhibition zone was observed for NiCTAC Ns and smallest for NiHEXA Ns which is related to maximum and minimum bactericidal properties of Ns, respectively. The interaction behavior of bacterial DNA with Ni nanosuspension was analyzed using agarose gel electrophoresis and circular dichroism. Along with, the role of different chemical scavengers was also evaluated in DNA damage using gel electrophoresis. Furthermore, the antioxidant activity of Ni nanosuspension was also confirmed using DPPH assay.
在本研究中，我们使用微乳液技术合成了氢氧化镍纳米悬浮液（Ns）。这种方法环保，利用吐温 80（一种非离子型生物相容表面活性剂）和新合成的金属表面活性剂，在纳米悬浮液（Ns）的形式下形成均匀的纳米颗粒。镍氢氧化物 Ns 来自三种不同的金属表面活性剂，即 NiCTAC（双十六烷基三甲基氯化铵镍四氯化物）、NiDDA（双十二烷基胺镍二氯化物）和 NiHEXA（双十六烷基胺镍二氯化物）。三种不同的镍基金属表面活性剂通过 CHN、HNMR 和 FTIR 等多种方法进行合成和表征。通过透射电子显微镜（TEM）、场发射扫描电子显微镜（FESEM）、能量色散 X 射线能谱（EDX）、X 射线衍射（XRD）、pH 值和 Zeta 电位等不同的表征方法证实了纳米悬浮液的制备。这些颗粒进一步研究了其对革兰氏阴性菌、大肠杆菌（E. coli）的遗传毒性和细胞毒性。通过平板计数法、琼脂孔扩散法和革兰氏染色法证实了纳米悬浮液对 E. coli 的影响。通过平板计数法，证实了纳米悬浮液对 E. coli 细胞集落形成能力的影响。琼脂孔扩散法提供了抗菌活性的估计，NiCTAC Ns 的抑菌圈最大，NiHEXA Ns 的抑菌圈最小，这分别与 Ns 的最大和最小杀菌性能有关。用琼脂糖凝胶电泳和圆二色性分析了细菌 DNA 与 Ni 纳米悬浮液的相互作用行为。同时，还通过凝胶电泳评价了不同化学清除剂在 DNA 损伤中的作用。此外，还通过 DPPH 法证实了 Ni 纳米悬浮液的抗氧化活性。
