Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Faculty of Medicine, Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.
Eur J Clin Microbiol Infect Dis. 2018 Feb;37(2):255-263. doi: 10.1007/s10096-017-3124-3. Epub 2017 Nov 4.
The humoral immune responses against 46 different staphylococcal antigens in 27 bacteremia patients infected by clonally related methicillin-resistant Staphylococcus aureus (MRSA) strains of a single sequence type (ST) 239 were investigated. A group of non-infected patients (n = 31) hospitalized for different reasons served as controls. All strains were confirmed as ST 239 by S. aureus and mecA-specific PCR, spa, and multi-locus sequence typing (MLST). In each bacteremia patient, a unique pattern of S. aureus antigen-specific immune responses after infection was observed. Antibody levels among bacteremia patients were significantly higher than controls for HlgB (P = 0.001), LukD (P = 0.009), LukF (P = 0.0001), SEA (P = 0.0001), SEB (P = 0.011), SEC (P = 0.010), SEQ (P = 0.049), IsaA (P = 0.043), IsdA (P = 0.038), IsdH (P = 0.01), SdrD (P = 0.001), SdrE (P = 0.046), EsxA (P = 0.0001), and SA0104 (P = 0.0001). On the other hand, the antibody levels were significantly higher among controls for SSL3 (P = 0.009), SSL9 (P = 0.002), and SSL10 (P = 0.007) when the IgG level on the day of infection was compared with that measured on the day of admission. Diversity was observed in the immune response against the antigens. However, a set of antigens (IsaA, IsdA, IsdH, SdrD, and HlgB) triggered a similar type of immune response in different individuals. We suggest that these antigens could be considered when developing a multi-component (passive) vaccine. SEA and/or its specific antibodies seem to play a critical role during ST239 MRSA bacteremia and SEA-targeted therapy may be a strategy to be considered.
对 27 例由单一序列型（ST）239 克隆相关耐甲氧西林金黄色葡萄球菌（MRSA）菌株引起的菌血症患者的 46 种不同葡萄球菌抗原的体液免疫反应进行了研究。一组因不同原因住院的非感染患者（n=31）作为对照组。所有菌株均通过金黄色葡萄球菌和 mecA 特异性 PCR、spa 和多位点序列分型（MLST）确认为 ST239。在每个菌血症患者中，观察到感染后金黄色葡萄球菌抗原特异性免疫反应的独特模式。与对照组相比，菌血症患者的抗体水平显著升高：HlgB（P=0.001）、LukD（P=0.009）、LukF（P=0.0001）、SEA（P=0.0001）、SEB（P=0.011）、SEC（P=0.010）、SEQ（P=0.049）、IsaA（P=0.043）、IsdA（P=0.038）、IsdH（P=0.01）、SdrD（P=0.001）、SdrE（P=0.046）、EsxA（P=0.0001）和 SA0104（P=0.0001）。另一方面，与入院当天相比，感染当天 IgG 水平的比较中，对照组的 SSL3（P=0.009）、SSL9（P=0.002）和 SSL10（P=0.007）的抗体水平更高。针对抗原的免疫反应存在多样性。然而，一组抗原（IsaA、IsdA、IsdH、SdrD 和 HlgB）在不同个体中引发了类似类型的免疫反应。我们建议在开发多组分（被动）疫苗时可以考虑这些抗原。SEA 和/或其特异性抗体似乎在 ST239 耐甲氧西林金黄色葡萄球菌菌血症中发挥关键作用，SEA 靶向治疗可能是一种需要考虑的策略。
