β-1,3-葡聚糖酶破坏白色念珠菌 DAY185 的生物膜形成并增加其抗真菌敏感性。
β-1,3-glucanase disrupts biofilm formation and increases antifungal susceptibility of Candida albicans DAY185.
机构信息
Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria.
Food Biotechnology Laboratory, Department of Food Sciences and Technology, BOKU-University of Natural Resources and Life Sciences, 1190 Vienna, Austria.
出版信息
Int J Biol Macromol. 2018 Mar;108:942-946. doi: 10.1016/j.ijbiomac.2017.11.003. Epub 2017 Nov 4.
β-1,3-glucan plays a role in Candida biofilm formation and survival of biofilm-forming Candida to stresses. In this study, we evaluated the antibiofilm activity of β-1,3-glucanase, which can degrade poly-β(1→3)-glucose of Candida albicans biofilms. Biofilm was dispersed by 55.96%. β-1,3-glucanase had no effect on Candida planktonic growth as well as adhesion. β-1,3-glucanase markedly enhanced the antifungal susceptibility of fluconazole and amphotericin B. The examination using confocal laser scanning microscopy and scanning electron microscope confirmed the antibiofilm activity of β-1,3-glucanase. Our findings demonstrate that β-1,3-glucanase may be useful as an antibiofilm agent.
β-1,3-葡聚糖在念珠菌生物膜形成和生物膜形成念珠菌对压力的存活中发挥作用。在这项研究中，我们评估了β-1,3-葡聚糖酶的抗生物膜活性，β-1,3-葡聚糖酶可以降解白色念珠菌生物膜中的聚-β(1→3)-葡萄糖。生物膜分散率为 55.96%。β-1,3-葡聚糖酶对念珠菌浮游生长和黏附没有影响。β-1,3-葡聚糖酶显著增强了氟康唑和两性霉素 B 的抗真菌敏感性。共聚焦激光扫描显微镜和扫描电子显微镜的检查证实了β-1,3-葡聚糖酶的抗生物膜活性。我们的研究结果表明，β-1,3-葡聚糖酶可能是一种有用的抗生物膜剂。
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