Terada N, Yamamoto R, Uchida N, Takada T, Taniguchi H, Takatsuka D, Sawada M, Tsuji M, Li W, Kitamura Y
Department of Pathology, Center for Adult Diseases Osaka, Japan.
Cancer Res. 1989 Feb 1;49(3):693-8.
An androgen-dependent tumor (SCC8 tumor) was obtained by inoculating an androgen-responsive cell line derived from the androgen-responsive Shionogi carcinoma 115 (SC115) into mice and then treating the mice with testosterone propionate (TP) at a pharmacological dose (400 micrograms/day). The SCC8 tumor differed in histological appearance from the SC115 tumor and its growth was less stimulated by androgen than that of the SC115 tumor. However, its growth was completely androgen dependent; SCC8 tumors did not develop in castrated mice and regressed when TP treatment was discontinued. The decreased sensitivity of the SCC8 tumor seemed to be attributable in part to its rapid metabolism of testosterone to metabolites with lower androgenic actions. The effects of TP at doses of 0, 100, 200, and 400 micrograms/day on cell division and cell death in SCC8 tumors of medium size were examined by measurements of the mitotic index and the retention of 5-[125I]iodo-2'-deoxyuridine incorporated into the whole tumor. TP increased the mitotic index dose dependently and at all doses reduced the decrease in the retention of 5-[125I]iodo-2'-deoxyuridine. These results suggest that steroids may not only stimulate cell division but also reduce cell death in steroid-dependent tumors.
通过将源自雄激素反应性的狮王制药癌115（SC115）的雄激素反应性细胞系接种到小鼠体内，然后以药理剂量（400微克/天）用丙酸睾酮（TP）处理小鼠，获得了一种雄激素依赖性肿瘤（SCC8肿瘤）。SCC8肿瘤在组织学外观上与SC115肿瘤不同，其生长受雄激素刺激的程度低于SC115肿瘤。然而，其生长完全依赖雄激素；去势小鼠中不会发生SCC8肿瘤，停止TP治疗后肿瘤会消退。SCC8肿瘤敏感性降低似乎部分归因于其将睾酮快速代谢为雄激素作用较低的代谢产物。通过测量有丝分裂指数和掺入整个肿瘤中的5-[125I]碘-2'-脱氧尿苷的保留情况，研究了0、100、200和400微克/天剂量的TP对中等大小SCC8肿瘤细胞分裂和细胞死亡的影响。TP剂量依赖性地增加有丝分裂指数，并且在所有剂量下都减少了5-[125I]碘-2'-脱氧尿苷保留量的下降。这些结果表明，类固醇不仅可能刺激细胞分裂，还可能减少类固醇依赖性肿瘤中的细胞死亡。
