环状RNA分析揭示chi_circ_0008219在山羊（Capra hircus）排卵前卵巢卵泡中作为微小RNA海绵的功能。
Circular RNA profiling reveals chi_circ_0008219 function as microRNA sponges in pre-ovulatory ovarian follicles of goats (Capra hircus).
作者信息
Tao Hu, Xiong Qi, Zhang Feng, Zhang Nian, Liu Yang, Suo Xiaojun, Li Xiaofeng, Yang Qianping, Chen Mingxin
机构信息
Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei, Academy of Agricultural Sciences, Wuhan 430064, China.
Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei, Academy of Agricultural Sciences, Wuhan 430064, China.
出版信息
Genomics. 2017 Oct 26. doi: 10.1016/j.ygeno.2017.10.005.
Circular RNAs (circRNAs) are a new class of non-coding RNAs in animals and are a novel target of non-coding RNA (ncRNA) regulation. The mechanism and function of circRNAs have been reported in some species and tissues. However, there is little available information on the functions of circRNAs in the goat reproductive system. In the present study, we deeply sequenced and analyzed circRNAs through bioinformatics to reveal the expression profiles, and predicted 13,950 circRNAs in the pre-ovulatory ovarian follicles of goats for the first time. Thirty-seven circRNAs were differentially expressed in the Boer goat compared with the Macheng black goat. The chi_circ_0008219 was involved in a vast circRNA-miRNA-mRNA co-expression network. Via a luciferase activity assay, chi_circ_0008219 is observed to sponge to 3 ovarian follicle-related miRNAs. These findings demonstrate that circRNAs have potential effects in the ovarian follicles of ewes and may represent a promising new research field in ovarian follicular development.
环状RNA（circRNAs）是动物体内一类新的非编码RNA，是一种新型的非编码RNA（ncRNA）调控靶点。circRNAs的机制和功能已在一些物种和组织中有所报道。然而，关于circRNAs在山羊生殖系统中的功能，目前可用信息较少。在本研究中，我们通过生物信息学对circRNAs进行了深度测序和分析，以揭示其表达谱，并首次在山羊排卵前卵巢卵泡中预测了13950个circRNAs。与麻城黑山羊相比，波尔山羊中有37个circRNAs差异表达。chi_circ_0008219参与了一个庞大的circRNA-miRNA-mRNA共表达网络。通过荧光素酶活性测定，观察到chi_circ_0008219可吸附3种与卵巢卵泡相关的miRNA。这些发现表明，circRNAs在母羊卵巢卵泡中具有潜在作用，可能代表了卵巢卵泡发育中一个有前景的新研究领域。
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