闭锁卵泡中高表达的circRALGPS2通过编码一种新蛋白诱导鸡颗粒细胞凋亡和自噬。

High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein.

作者信息

He Haorong, Wei Yuanhang, Chen Yuqi, Zhao Xiyu, Shen Xiaoxu, Zhu Qing, Yin Huadong

机构信息

Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.

出版信息

J Anim Sci Biotechnol. 2024 Mar 11;15(1):42. doi: 10.1186/s40104-024-01003-w.

BACKGROUND

The reproductive performance of chickens mainly depends on the development of follicles. Abnormal follicle development can lead to decreased reproductive performance and even ovarian disease among chickens. Chicken is the only non-human animal with a high incidence of spontaneous ovarian cancer. In recent years, the involvement of circRNAs in follicle development and atresia regulation has been confirmed.

RESULTS

In the present study, we used healthy and atretic chicken follicles for circRNA RNC-seq. The results showed differential expression of circRALGPS2. It was then confirmed that circRALGPS2 can translate into a protein, named circRALGPS2-212aa, which has IRES activity. Next, we found that circRALGPS2-212aa promotes apoptosis and autophagy in chicken granulosa cells by forming a complex with PARP1 and HMGB1.

CONCLUSIONS

Our results revealed that circRALGPS2 can regulate chicken granulosa cell apoptosis and autophagy through the circRALGPS2-212aa/PARP1/HMGB1 axis.

背景

鸡的繁殖性能主要取决于卵泡的发育。卵泡发育异常会导致鸡的繁殖性能下降，甚至引发卵巢疾病。鸡是唯一一种自发性卵巢癌发病率较高的非人类动物。近年来，环状RNA在卵泡发育和闭锁调节中的作用已得到证实。

结果

在本研究中，我们使用健康和闭锁的鸡卵泡进行环状RNA核糖体印迹测序。结果显示环状RALGPS2存在差异表达。随后证实环状RALGPS2可以翻译为一种名为circRALGPS2-212aa的蛋白质，该蛋白质具有内部核糖体进入位点（IRES）活性。接下来，我们发现circRALGPS2-212aa通过与PARP1和HMGB1形成复合物，促进鸡颗粒细胞的凋亡和自噬。