激光在植入前基因诊断中成功应用于人类胚胎活检：两例报告。
Successful use of a laser for human embryo biopsy in preimplantation genetic diagnosis: report of two cases.
作者信息
Boada M, Carrera M, De La Iglesia C, Sandalinas M, Barri P N, Veiga A
机构信息
Departament d'Obstetrícia i Ginecologia, Institut Universitari Dexeus, Barcelona, Spain.
出版信息
J Assist Reprod Genet. 1998 May;15(5):302-7. doi: 10.1023/a:1022548612107.
Abstract
PURPOSE
The use of Tyrode's acid to drill the zona pellucida for embryo biopsy is the most widely used methodology in preimplantation genetic diagnosis. Instead of this, we propose the use of a 1.48-micron diode noncontact laser, which is quicker, simpler, and safer.
METHODS
The laser beam was tangentially guided to the zona pellucida of the embryo. Depending on zona pellucida measurement, two to four consecutive shots of 8-22 msec were necessary to drill the zona pellucida of the 13 embryos biopsied for two patients (hemophilia carriers).
RESULTS
Female embryos were replaced into the uterus of the patients (1.5 embryos/replacement). One single pregnancy was established (33.3% implantation rate). Coculture of untransferable embryos showed a blastocyst rate of 66.7% (4/6) for male embryos and 25% (1/4) for abnormal ones.
CONCLUSIONS
These results demonstrate the safety and usefulness of laser methodology in preimplantation genetic diagnosis.
摘要
目的
在植入前基因诊断中，使用台氏酸钻透透明带进行胚胎活检是应用最广泛的方法。我们提出使用1.48微米二极管非接触式激光替代该方法，其操作更快、更简单且更安全。
方法
将激光束沿切线方向导向胚胎的透明带。根据透明带的测量结果，对为两名患者（血友病携带者）活检的13个胚胎钻透透明带，需要连续进行2至4次8 - 22毫秒的照射。
结果
将雌性胚胎移植到患者子宫（每次移植1.5个胚胎）。成功建立了单胎妊娠（着床率33.3%）。对未移植胚胎进行共培养显示，雄性胚胎的囊胚率为66.7%（4/6），异常胚胎的囊胚率为25%（1/4）。
结论
这些结果证明了激光方法在植入前基因诊断中的安全性和实用性。
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