Oliveira Fernanda de Sousa, Freitas Thiago Sampaio de, Cruz Rafael Pereira da, Costa Maria do Socorro, Pereira Raimundo Luiz Silva, Quintans-Júnior Lucindo José, Andrade Tatianny de Araújo, Menezes Paula Dos Passos, Sousa Bruna Maria Hipólito de, Nunes Paula Santos, Serafini Mairim Russo, Menezes Irwin Rose Alencar de, Araújo Adriano Antunes de Souza, Coutinho Henrique Douglas Melo
Microbiology and Molecular Biology Laboratory, Regional University of Cariri, Crato, Ceará, Brazil.
Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil.
Biomed Pharmacother. 2017 Aug;92:1111-1118. doi: 10.1016/j.biopha.2017.06.020. Epub 2017 Jun 12.
The present study aimed to evaluate the antibacterial and modulatory potential of α-bisabolol, β-cyclodextrin and α-bisabolol/β-cyclodextrin complex. The minimum inhibitory concentration was determined through the broth microdilution technique using the bacterial strains: Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The drugs norfloxacin, imipenem and gentamicin were used in the tests, and the compounds α-bisabolol and β-cyclodextrin; all the compounds were diluted in DMSO. To obtain the minimum inhibitory concentration (MIC) a serial microdilution of the substances in volumes corresponding to the sub-inhibitory concentration (MIC/8), and microdilution with the antibiotic until the penultimate well were performed. The results showed that β-cyclodextrin did not present synergistic effects when combined with the antibiotics. It was found that α-bisabolol presented a synergistic effect against S. aureus, when combined with the antibiotic norfloxacin. Moreover, α-bisabolol presented synergism against E. coli when combined with gentamicin. The results of this study show that α-bisabolol presents a modulatory synergistic effect for some antibiotics, as gentamicin, and this is an interesting result against multidrug resistant bacteria (MDR). By other side, the complexation of α-bisabolol with β-cyclodextrin apparently reduces the modulatory effect, maybe due the polarity enhancement of the polarity of α-bisabolol, affecting the interaction of this compound with the cell membrane bilayer. However, more studies are necessary to demonstrate or not these interactions.
本研究旨在评估红没药醇、β-环糊精及红没药醇/β-环糊精复合物的抗菌及调节潜力。采用肉汤微量稀释技术，以金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌为菌株，测定最低抑菌浓度。试验中使用了诺氟沙星、亚胺培南和庆大霉素等药物，以及红没药醇和β-环糊精这两种化合物；所有化合物均用二甲亚砜稀释。为获得最低抑菌浓度（MIC），将物质进行系列微量稀释，使其体积对应亚抑菌浓度（MIC/8），并与抗生素进行微量稀释直至倒数第二孔。结果表明，β-环糊精与抗生素联合使用时未呈现协同作用。研究发现，红没药醇与抗生素诺氟沙星联合使用时，对金黄色葡萄球菌呈现协同作用。此外，红没药醇与庆大霉素联合使用时，对大肠杆菌呈现协同作用。本研究结果表明，红没药醇对某些抗生素如庆大霉素呈现调节协同作用，这对于多重耐药菌（MDR）来说是一个有趣的结果。另一方面，红没药醇与β-环糊精的络合作用显然降低了调节效果，这可能是由于红没药醇极性增强，影响了该化合物与细胞膜双层的相互作用。然而，需要更多的研究来证实或否定这些相互作用。
