Khoo S K, Hurst T, Lark J, Webb M J, Parsons P, Mackay E V
University of Queensland, Department of Obstetrics and Gynaecology, Royal Brisbane Hospital, Australia.
Gynecol Oncol. 1989 Feb;32(2):151-5. doi: 10.1016/s0090-8258(89)80026-5.
Tumor sensitivity of ovarian cancer to cytotoxic drugs in vitro was examined between paired samples taken from the primary tumor and its metastases in the same patient. A 3-hour assay of primary cultures of the tumor was used to determine cellular response to the drugs by measurement of the incorporation of [3H]thymidine. Although there was variability, a reasonable correlation was found between the primary tumor and its different metastases, between the different metastases, and between the different tumor sites and cells from the peritoneal fluid. However, there was discordance between some of the pairings; the rate varied between 12 and 42% when the criterion was greater than 20% inhibition. Consistent drug sensitivity for all sites tested was present in only 8 of the 18 patients. Because of the discordance in some pairings, attempts should be made to sample all metastatic tumors, particularly those which cannot be removed completely by surgery. The discordant subgroup may be a source of false-negative and false-positive results of the assay.
在同一患者的原发性肿瘤及其转移灶的配对样本之间，检测了卵巢癌对细胞毒性药物的体外敏感性。通过测量[3H]胸腺嘧啶核苷的掺入量，对肿瘤原代培养物进行3小时检测，以确定细胞对药物的反应。尽管存在变异性，但在原发性肿瘤与其不同转移灶之间、不同转移灶之间以及不同肿瘤部位与腹水细胞之间发现了合理的相关性。然而，一些配对之间存在不一致；当标准为抑制率大于20%时，不一致率在12%至42%之间。18例患者中只有8例所有检测部位均呈现一致的药物敏感性。由于一些配对存在不一致，应尝试对所有转移性肿瘤进行取样，尤其是那些无法通过手术完全切除的肿瘤。不一致的亚组可能是检测假阴性和假阳性结果的来源。
