School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China.
School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou 510006, PR China.
Sci Total Environ. 2018 Mar;616-617:107-116. doi: 10.1016/j.scitotenv.2017.10.224. Epub 2017 Nov 4.
Lacking sufficient clean water, the paddy soils along the Hengshi River have suffered from long-term acid mine drainage (AMD) contamination. The impacted cropland is too heavily contaminated to grow food safely. The microbial communities inhabiting the environment play pivotal roles in the crop growth, health, and ecological services. In this study, the bacterial, archaeal, and fungal communities in the impacted paddy soil were examined using high-throughput Illumina MiSeq sequencing. The results showed that AMD irrigation considerably enriched the bacterial phylum Acidobacteria and the archaeal phylum Crenarchaeota, while the fungal community was more stable. The abundances of Acidobacteria and Crenarchaeota were significantly positively correlated with the AMD-related environmental factors of pH and heavy metals (Cu, Pb, and Zn). In the most contaminated samples, communities were dominated by the bacteria Candidatus Solibacter and Candidatus Koribacter from the Acidobacteria family. Functional gene profile analysis demonstrated that the energy metabolic processes of the microbial communities, especially C/N related pathways, have adjusted and are well-adapted to tolerating AMD contamination. The present study described the structural and functional differentiation of microbial communities in the rice paddy soil under AMD irrigation. The results are useful for the development of bioremediation strategies using native microbes in the cleanup and biorestoration of AMD-contaminated agriculture soil.
由于缺乏足够的清洁水，横石河流域的稻田土壤长期受到酸性矿山排水（AMD）的污染。受影响的农田污染太严重，无法安全种植粮食。栖息在环境中的微生物群落对作物生长、健康和生态服务起着关键作用。在这项研究中，使用高通量 Illumina MiSeq 测序技术研究了受影响稻田土壤中的细菌、古菌和真菌群落。结果表明，AMD 灌溉显著富集了细菌门酸杆菌和古菌门泉古菌门，而真菌群落则更稳定。酸杆菌门和泉古菌门的丰度与 AMD 相关的环境因子 pH 和重金属（Cu、Pb 和 Zn）呈显著正相关。在污染最严重的样本中，群落主要由酸杆菌门的候选 Solibacter 和候选 Koribacter 细菌主导。功能基因谱分析表明，微生物群落的能量代谢过程，特别是 C/N 相关途径，已经进行了调整，并且很好地适应了 AMD 污染。本研究描述了 AMD 灌溉下稻田土壤中微生物群落的结构和功能分化。研究结果有助于开发利用本地微生物进行 AMD 污染农业土壤修复和生物修复的生物修复策略。
