Lacombe M J, Delarue J C, Mouriesse H, Contesso G, Friedman S, Sancho-Garnier H, Travagli J P, May-Levin F
Department of Pathology, Institut Gustave-Roussy, Villejuif, France.
Gynecol Oncol. 1989 Feb;32(2):174-9. doi: 10.1016/s0090-8258(89)80029-0.
In a series of 94 human mammary carcinomas, the determination of total estrogen (ER) and progesterone (PR) receptors by a single saturating dose method (5 nM for ER, 10 nM for PR) using dextran-coated charcoal was compared to an immunohistochemical method utilizing ER monoclonal antibody (ER-ICA test). There was a good correlation expressed in positive terms between the ER-ICA test and the biochemical determination of ER (94% of concordance) with a statistical value of P less than 0.01 being found between the concentration of ER (biochemical) and the percentage of labeled cells (ER-ICA). The ER-ICA test complements the ER and PR (biochemical) and is particularly useful for ER determinations on small tumor specimens as no additional tissue other than that from the biopsy is required.
在一组94例人类乳腺癌中，采用葡聚糖包被活性炭的单饱和剂量法（雌激素受体[ER]为5 nM，孕激素受体[PR]为10 nM）测定总雌激素受体和孕激素受体，并与使用ER单克隆抗体的免疫组织化学方法（ER免疫细胞化学检测）进行比较。ER免疫细胞化学检测与ER的生化测定之间呈良好的正相关（一致性为94%），ER（生化）浓度与标记细胞百分比（ER免疫细胞化学检测）之间的统计值P小于0.01。ER免疫细胞化学检测对ER和PR（生化）检测起到补充作用，尤其适用于小肿瘤标本的ER测定，因为除活检组织外无需额外组织。
