Feng Xu, Fang Xue, Xia Lingzi, Ren Yangwu, Li Xuelian, Quan Xiaowei, Zhou Baosen
Department of Epidemiology, School of Public Health, China Medical University, Liaoning, China.
Department of Health Statistics, School of Public Health, Shenyang Medical College, Liaoning, China.
Oncotarget. 2017 Aug 12;8(45):78749-78756. doi: 10.18632/oncotarget.20224. eCollection 2017 Oct 3.
PPP1R13L and CD3EAP were confirmed to play important roles in transcription and apoptosis. SNPs in PPP1R13L and CD3EAP may be associated with lung cancer risk and survival. This study investigated the association of PPP1R13L rs1005165 and CD3EAP rs967591 with non-small cell lung cancer (NSCLC) risk and survival in Chinese non-smoking females. 442 NSCLC cases and 480 cancer-free controls were conducted in the case-control study, and 283 cases were in cohort study. Genotype was determined by Taqman real-time PCR. The statistical analyses were performed by SPSS 22.0 software. We found that rs1005165 and rs967591 were significantly associated with NSCLC risk in Chinese non-smoking females. For rs1005165, compared with homozygous wild CC genotype, carriers of CT or TT genotype had lower risk of NSCLC (adjusted ORs were 0.675 and 0.713, 95% CI were 0.461-0.988 and 0.525-0.968, respectively), adjusted OR for dominant model was 0.702, 95% CI was 0.526-0.937. For rs967591, AA genotype (adjusted OR = 0.721, 95% CI = 0.532-0.978) and at least one A allele (GA+AA) (adjusted OR = 0.716, 95% CI = 0.536-0.956) were significantly correlated with lower risk of NSCLC, compared with GG genotype. But we didn't find correlation between the two SNPs and survival time in Chinese non-smoking NSCLC females. In general, we found PPP1R13L rs1005165 and CD3EAP rs967591 might be associated with lower NSCLC risk in Chinese non-smoking females, but no significant relationship was found with NSCLC survival.
PPP1R13L和CD3EAP被证实可在转录和细胞凋亡过程中发挥重要作用。PPP1R13L和CD3EAP中的单核苷酸多态性（SNP）可能与肺癌风险及生存率相关。本研究调查了PPP1R13L基因rs1005165位点和CD3EAP基因rs967591位点与中国非吸烟女性非小细胞肺癌（NSCLC）风险及生存率之间的关联。病例对照研究纳入了442例NSCLC病例和480例无癌对照，队列研究纳入了283例病例。采用Taqman实时荧光定量PCR法确定基因型。运用SPSS 22.0软件进行统计分析。我们发现rs1005165和rs967591与中国非吸烟女性的NSCLC风险显著相关。对于rs1005165位点，与纯合野生型CC基因型相比，CT或TT基因型携带者患NSCLC的风险较低（校正后的比值比分别为0.675和0.713，95%置信区间分别为0.461 - 0.988和0.525 - 0.968），显性模型的校正后比值比为0.702，95%置信区间为0.526 - 0.937。对于rs967591位点,与GG基因型相比,AA基因型（校正后的比值比 = 0.721, 95%置信区间 = 0.532 - 0.978）以及至少携带一个A等位基因（GA + AA）（校正后的比值比 = 0.716, 95%置信区间 = 0.536 - 0.956）与较低的NSCLC风险显著相关。但我们未发现这两个SNP与中国非吸烟NSCLC女性的生存时间存在相关性。总体而言，我们发现PPP1R13L基因rs1005165位点和CD3EAP基因rs967591位点可能与中国非吸烟女性较低的NSCLC风险相关，但与NSCLC生存率无显著关联。
