Ries-Kautt M M, Ducruix A F
Laboratoire de Cristallochimie, Centre National de la Recherche Scientifique, Gif sur Yvette, France.
J Biol Chem. 1989 Jan 15;264(2):745-8.
Crystallization conditions for hen egg white lysozyme in the presence of various ions were determined at pH 4.5 and 18 degrees C. The corresponding solubility curves show that the main effects are due to anions in the following order: SCN- greater than NO3- greater than Cl- greater than citrate2- greater than CH3COO- approximately H2PO4- greater than SO4(2-). This is in the reverse order of the lyotropic series of Hofmeister. As a consequence, SCN- precipitates and crystallizes lysozyme at low concentration, whereas sulfate is ineffective even at high concentrations. Crystals obtained with each salt were characterized by x-ray diffraction. Lysozyme thiocyanate and nitrate crystals belong to the monoclinic system, whereas all the others have a tetragonal lattice.
在pH值为4.5和18摄氏度的条件下，测定了在各种离子存在时鸡蛋清溶菌酶的结晶条件。相应的溶解度曲线表明，主要影响因素是阴离子，其顺序如下：硫氰酸根离子＞硝酸根离子＞氯离子＞柠檬酸根离子＞醋酸根离子≈磷酸二氢根离子＞硫酸根离子。这与霍夫迈斯特感胶离子序相反。因此，硫氰酸根离子在低浓度时就能使溶菌酶沉淀并结晶，而硫酸根离子即使在高浓度时也无效。用每种盐获得的晶体通过X射线衍射进行了表征。溶菌酶硫氰酸盐和硝酸盐晶体属于单斜晶系，而其他所有晶体都具有四方晶格。
