Copeland Robert L, Beyene Desta, Apprey Victor, Daremipouran Mohammad R, Naab Tammey J, Kassim Olakunle O, Kanaan Yasmine M
Department of Pharmacology, Howard University, Washington, DC, U.S.A.
Cancer Center, Howard University, Washington, DC, U.S.A.
Cancer Genomics Proteomics. 2017 Nov-Dec;14(6):461-467. doi: 10.21873/cgp.20056.
BACKGROUND/AIM: Denaturing high-performance liquid chromatography (DHPLC) is a technique that is used to detect mutations. The aim of the present study was to determine whether DHPLC elution patterns of vitamin D receptor (VDR) gene PCR products can serve as indicators of susceptibility to prostate cancer (PCa) risk.
DNA samples of PCa cases and controls were screened for mutations and/or polymorphisms in coding exons of VDR gene using DHPLC analysis. Logistic regression, phi-coefficient (ϕ), and Backward Wald models were used to analyze the data.
Similar elution patterns of exons 1, 6, 7 and 9 along with higher prevalence of heteroduplex DNA were observed in PCa samples than in controls. Exons 4 and 8 had highly significant protective effects (p<0.05). Whereas, exons 5, 7, and 9 were perfectly positively correlated with PCa risk (ϕ=1), thus presenting candidate exons significantly associated with susceptibility to PCa.
DHPLC elution patterns of the selected exons could be useful to predict susceptibility to develop PCa.
背景/目的：变性高效液相色谱法（DHPLC）是一种用于检测突变的技术。本研究的目的是确定维生素D受体（VDR）基因PCR产物的DHPLC洗脱模式是否可作为前列腺癌（PCa）风险易感性的指标。
使用DHPLC分析对PCa病例和对照的DNA样本进行VDR基因编码外显子的突变和/或多态性筛查。采用逻辑回归、phi系数（ϕ）和向后Wald模型分析数据。
与对照相比，在PCa样本中观察到外显子1、6、7和9的洗脱模式相似，同时异源双链DNA的患病率更高。外显子4和8具有高度显著的保护作用（p<0.05）。而外显子5、7和9与PCa风险呈完全正相关（ϕ=1），因此呈现出与PCa易感性显著相关的候选外显子。
所选外显子的DHPLC洗脱模式可能有助于预测发生PCa 的易感性。
