聚合酶链反应产物的变性高效液相色谱洗脱模式可预测非裔美国男性患前列腺癌的易感性。
DHPLC Elution Patterns of PCR Products Can Predict Prostate Cancer Susceptibility in African American Men.
作者信息
Copeland Robert L, Beyene Desta, Apprey Victor, Daremipouran Mohammad R, Naab Tammey J, Kassim Olakunle O, Kanaan Yasmine M
机构信息
Department of Pharmacology, Howard University, Washington, DC, U.S.A.
Cancer Center, Howard University, Washington, DC, U.S.A.
出版信息
Cancer Genomics Proteomics. 2017 Nov-Dec;14(6):461-467. doi: 10.21873/cgp.20056.
BACKGROUND/AIM: Denaturing high-performance liquid chromatography (DHPLC) is a technique that is used to detect mutations. The aim of the present study was to determine whether DHPLC elution patterns of vitamin D receptor (VDR) gene PCR products can serve as indicators of susceptibility to prostate cancer (PCa) risk.
MATERIALS AND METHODS
DNA samples of PCa cases and controls were screened for mutations and/or polymorphisms in coding exons of VDR gene using DHPLC analysis. Logistic regression, phi-coefficient (ϕ), and Backward Wald models were used to analyze the data.
RESULTS
Similar elution patterns of exons 1, 6, 7 and 9 along with higher prevalence of heteroduplex DNA were observed in PCa samples than in controls. Exons 4 and 8 had highly significant protective effects (p<0.05). Whereas, exons 5, 7, and 9 were perfectly positively correlated with PCa risk (ϕ=1), thus presenting candidate exons significantly associated with susceptibility to PCa.
CONCLUSION
DHPLC elution patterns of the selected exons could be useful to predict susceptibility to develop PCa.
背景/目的：变性高效液相色谱法（DHPLC）是一种用于检测突变的技术。本研究的目的是确定维生素D受体（VDR）基因PCR产物的DHPLC洗脱模式是否可作为前列腺癌（PCa）风险易感性的指标。
材料与方法
使用DHPLC分析对PCa病例和对照的DNA样本进行VDR基因编码外显子的突变和/或多态性筛查。采用逻辑回归、phi系数（ϕ）和向后Wald模型分析数据。
结果
与对照相比，在PCa样本中观察到外显子1、6、7和9的洗脱模式相似，同时异源双链DNA的患病率更高。外显子4和8具有高度显著的保护作用（p<0.05）。而外显子5、7和9与PCa风险呈完全正相关（ϕ=1），因此呈现出与PCa易感性显著相关的候选外显子。
结论
所选外显子的DHPLC洗脱模式可能有助于预测发生PCa 的易感性。
Zotero 插件