Yokose Satoshi, Klokkevold Perry R, Takei Henry H, Kadokura Hiroshi, Kikui Tetsuya, Hibino Yasushi, Shigeta Hirotaka, Nakajima Hiroshi, Kawazu Hiroshi
Division of Endodontic and Operative Dentistry, Department of Restorative and Biomaterials Sciences, School of Dentistry, Meikai University.
Division of Constitutive and Regenerative Sciences, Section of Periodontics, School of Dentistry University California Los Angels.
Dent Mater J. 2018 Mar 30;37(2):272-277. doi: 10.4012/dmj.2017-036. Epub 2017 Nov 3.
The surface topography of implant fixture is an important factor affecting the osseointegration. We herein demonstrated the effects of surface microtopography of titanium disks on proliferation and differentiation of osteoblast-like cells isolated from rat calvariae. Titanium disks with machine surface (MS), rough surface (R1) and rough surface combined with small cavities (R2) were used in an in vitro culture system. Rough surfaces (R1 and R2 disks) induced stronger osteoblast proliferation and differentiation (BGP and sclerostin mRNA expressions and calcium content) than the smooth surface (MS disk). Furthermore, surface microtopography of R2 disk, which was rough with small cavities, more strongly induced cell proliferation and mineralized bone matrix production than R1 disk. Our results suggest that surface microtopography influences osteoblast proliferation and differentiation. R2 disk, which is rough with small cavities, may be used in implant fixtures to increase osseointegration.
种植体固定装置的表面形貌是影响骨整合的一个重要因素。我们在此展示了钛盘表面微观形貌对从大鼠颅骨分离的成骨样细胞增殖和分化的影响。在体外培养系统中使用了具有机械表面（MS）、粗糙表面（R1）和带有小腔的粗糙表面（R2）的钛盘。粗糙表面（R1和R2盘）比光滑表面（MS盘）诱导更强的成骨细胞增殖和分化（骨钙素和硬化蛋白mRNA表达以及钙含量）。此外，带有小腔的粗糙R2盘的表面微观形貌比R1盘更强烈地诱导细胞增殖和矿化骨基质产生。我们的结果表明，表面微观形貌影响成骨细胞的增殖和分化。带有小腔的粗糙R2盘可用于种植体固定装置以增加骨整合。
