Siberian Federal University, 79, Svobodnyav, Krasnoyarsk 660041, Russia.
L.V. Kirensky Institute of Physics, Siberian Branch of the Russian Academy of Sciences, 50/38 Akademgorodok, Krasnoyarsk 660036, Russia.
Biomolecules. 2019 Dec 30;10(1):65. doi: 10.3390/biom10010065.
The effect of surface relief profiles of alkanoate-based bionanofilms to the monocyte-macrophages (MN-MPhs) from peripheral blood of patients with atherosclerosis was studied in vitro. Patients were subjected to coronary stenting. Cell morphology and phenotype (expression of CD antigens, levels of production of marker cytokines) in vitro were analyzed before and after the installation of stents. It was shown, that the mean square roughness (Rq) of the bionanofilms determined the variability of cell morphology, CD antigens spectraand activity of production interleukins-6 and -10. Also, it was revealed, that the "activity" of the surface topography of biopolymer substrates depends on the functional state of MNs, isolated in different time points: Before and after stenting the ratios of cell morphotypes and production of cytokines in MN-MPhs differed significantly.
研究了基于烷酸酯的生物纳滤膜表面形貌对动脉粥样硬化患者外周血单核细胞-巨噬细胞（MN-MPhs）的体外影响。这些患者接受了冠状动脉支架植入术。在支架植入前后，对体外细胞形态和表型（CD 抗原表达、标记细胞因子的产生水平）进行了分析。结果表明，生物纳滤膜的均方根粗糙度（Rq）决定了细胞形态、CD 抗原谱和白细胞介素-6 和 -10 产生活性的可变性。此外，还揭示了生物聚合物基底表面形貌的“活性”取决于在不同时间点分离的 MN 的功能状态：支架植入前后，MN-MPhs 的细胞形态和细胞因子产生的比例有显著差异。
