College of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China.
Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea.
Molecules. 2017 Oct 29;22(11):1823. doi: 10.3390/molecules22111823.
In the present study, 16 marine-derived fungi were isolated from four types of marine materials including float, algae, animals and drift woods along with the coast of Jeju Island, Korea and evaluated for anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW 24.7 cells. The broth and mycelium extracts from the 16 fungi were prepared and the broth extract (BE) of (015-2) inhibited nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells without cytotoxicity. By further bioassay-guided isolation, three compounds including asperflavin, neoechinulin A and preechinulin were successfully isolated from the BE of . It was revealed that asperflavin showed no cytotoxicity up to 200 μM and significantly inhibited LPS-induced NO and PGE2 production in a dose-dependent manner. In the western blot results, asperflavin suppressed only inducible NOS (iNOS), but COX-2 were slightly down-regulated. Asperflavin was also observed to inhibit the production of pro-inflammatory cytokines including TNF-α, IL-1β, and IL-6. In conclusion, this study reports a potential use of asperflavin isolated from a marine fungus, as an anti-inflammatory agent via suppression of iNOS and pro-inflammatory cytokines as well as no cytotoxicity.
在本研究中，从韩国济州岛沿海的四种海洋材料（浮标、藻类、动物和漂流木）中分离出 16 株海洋来源真菌，并对其在脂多糖 (LPS) 刺激的 RAW 24.7 细胞中的抗炎作用进行评估。从 16 株真菌中制备了培养液和菌丝体提取物，并发现（015-2）的培养液提取物（BE）在无细胞毒性的情况下抑制 LPS 刺激的 RAW 264.7 细胞中的一氧化氮（NO）产生。通过进一步的生物活性导向分离，成功地从的 BE 中分离出三种化合物，包括asperflavin、neoechinulin A 和 preechinulin。结果表明，asperflavin 在高达 200 μM 的浓度下没有细胞毒性，并以剂量依赖的方式显著抑制 LPS 诱导的 NO 和 PGE2 产生。在 Western blot 结果中，asperflavin 仅抑制诱导型一氧化氮合酶 (iNOS)，但 COX-2 略有下调。asperflavin 还观察到抑制包括 TNF-α、IL-1β 和 IL-6 在内的促炎细胞因子的产生。总之，本研究报道了一种从海洋真菌中分离出的 asperflavin 作为抗炎剂的潜在用途，通过抑制 iNOS 和促炎细胞因子以及无细胞毒性。
