Lu Zhen-Ming, Zhu Qing, Li Hua-Xiang, Geng Yan, Shi Jin-Song, Xu Zheng-Hong
National Engineering Laboratory for Cereal Fermentation Technology, School of Pharmaceutical Science, Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, China.
Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
Front Microbiol. 2017 Oct 23;8:2048. doi: 10.3389/fmicb.2017.02048. eCollection 2017.
Wild fruiting bodies of medicinal mushroom are only found on the endemic species bull camphor tree, , in Taiwan. Despite the evident importance of the host components in promoting the growth of , insights into the underlying mechanisms are still lacking. Here, we first evaluated effects of the compounds from , and , and their structural analogs on the germination rate of arthroconidia. Among the 54 tested compounds, vanillin (4-hydroxy-3-methoxybenzaldehyde) was determined as the optimum germination promoter, while -vanillin and 1-octen-3-ol as major negative regulators of arthroconidia germination. Second, the protein patterns of arthroconidia after 24 h of incubation in the presence or absence of vanillin were compared via isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics. Via bioinformatic analysis, it was found that 61 proteins might relate to the germination of arthroconidia, in which 16 proteins might involve in two potential protein kinase A (PKA) and mitogen-activated protein kinase (MAPK) signaling pathways in the vanillin-promoted germination of arthroconidia. Last, the mRNA expression levels of the 16 germination-related genes in the potential PKA and MAPK signaling pathways were analyzed by quantitative real time PCR. Together, our results are beneficial for the elucidation of molecular mechanisms underlying the germination of arthroconidia.
药用蘑菇的野生子实体仅在台湾的特有物种牛樟树上发现。尽管宿主成分在促进其生长方面具有明显重要性，但对其潜在机制的了解仍然不足。在这里，我们首先评估了来自牛樟树、台湾扁柏及其结构类似物的化合物对节孢子萌发率的影响。在54种测试化合物中，香草醛（4-羟基-3-甲氧基苯甲醛）被确定为最佳萌发促进剂，而β-香草醛和1-辛烯-3-醇是节孢子萌发的主要负调节剂。其次，通过基于等压标签相对和绝对定量（iTRAQ）的蛋白质组学比较了在有或没有香草醛的情况下培养24小时后节孢子的蛋白质模式。通过生物信息学分析，发现61种蛋白质可能与节孢子的萌发有关，其中16种蛋白质可能参与了香草醛促进节孢子萌发的两条潜在蛋白激酶A（PKA）和丝裂原活化蛋白激酶（MAPK）信号通路。最后，通过定量实时PCR分析了潜在PKA和MAPK信号通路中16个与萌发相关基因的mRNA表达水平。总之，我们的结果有助于阐明节孢子萌发的分子机制。
