Department of Life Sciences, and Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan; Department of Biotechnology, Asia University, Taichung 413, Taiwan.
J Ethnopharmacol. 2015 Jan 15;159:113-21. doi: 10.1016/j.jep.2014.11.015. Epub 2014 Nov 18.
Antrodia camphorata, a highly valued polypore mushroom native only to Taiwan, has been traditionally used as a medicine for anti-inflammation.
In this study, anti-inflammatory effects of Antrodia camphorata (AC) and its active compound, ergostatrien-3β-ol (ST1), were investigated in a mouse skin ischemia model induced by skin flap surgery on the dorsal skin.
A U-shaped flap was elevated on the dorsal skin of the nine-week-old male mice. Mice were randomly assigned to six groups for treatment (n=6) including normal skin/propylene glycol (PG), surgical skin flap/PG, solid-state-cultured AC (S/AC), wood-cultured AC (W/AC), high-dose ST1 (H-ST1), low-dose ST1 (L-ST1). Antrodia camphorata was dissolved in 25μL PG and smeared on the skin flap every six hours for 24h. At the end of the experiment, each mouse was anesthetized, and skin tissues were collected from their back for histopathological analysis, extracting RNA and protein according to our previous reports.
Skin-flap-induced ischemia damage significantly increased the expression of the iNOS, COX2, and IL-6 proteins and decreased the expression of IκB protein. In addition, focal, moderate coagulative necrosis with inflammatory cell infiltration was found in the epidermis, and moderate inflammatory cells and necrosis with slight edema was noted in the sub-dermis at 24h after skin flap surgery. However, treatment with solid-state-cultured or wood-cultured AC, or with its derived ST1 active compound, significantly reduced the necrosis and inflammatory cell infiltration in both the epidermis and sub-dermis of the skin flap. The treatments also reduced the inflammatory response by decreasing the expression of inflammation-related genes including iNOS, IL-6, TNF-α, and NF-κB, as shown by changes in RNA and protein expression, when compared with the surgical skin flap procedure alone.
These results demonstrated that methanolic extracts of wood-cultured fruiting bodies and solid-state-cultured mycelia from Antrodia camphorata have excellent anti-inflammatory activities and thus have great potential as an addition for hydrocolloid dressings.
Antrodia camphorata 是一种高度珍贵的多孔菌，仅原产于中国台湾，传统上被用作抗炎药物。
本研究旨在探讨 Antrodia camphorata（AC）及其活性化合物 ergostatrien-3β-ol（ST1）在背部皮肤皮瓣手术后诱导的小鼠皮肤缺血模型中的抗炎作用。
在 9 周龄雄性小鼠背部皮肤上掀起 U 形皮瓣。将小鼠随机分为 6 组进行治疗（n=6），包括正常皮肤/丙二醇（PG）、手术皮瓣/PG、固态培养的 AC（S/AC）、木质培养的 AC（W/AC）、高剂量 ST1（H-ST1）、低剂量 ST1（L-ST1）。Antrodia camphorata 溶解在 25μL PG 中，每 6 小时涂抹在皮瓣上 24 小时。实验结束时，用麻醉剂麻醉每只小鼠，从背部采集皮肤组织进行组织病理学分析，根据我们之前的报告提取 RNA 和蛋白质。
皮瓣诱导的缺血损伤显著增加了 iNOS、COX2 和 IL-6 蛋白的表达，降低了 IκB 蛋白的表达。此外，皮瓣手术后 24 小时，在表皮中发现局灶性、中度凝固性坏死伴炎症细胞浸润，在真皮中发现中度炎症细胞和轻微水肿性坏死。然而，固态培养或木质培养的 AC 或其衍生的 ST1 活性化合物的治疗显著减少了皮瓣表皮和真皮中的坏死和炎症细胞浸润。通过改变 RNA 和蛋白质表达，与单独的手术皮瓣程序相比，这些治疗还减少了与炎症相关的基因（包括 iNOS、IL-6、TNF-α 和 NF-κB）的表达，从而减轻了炎症反应。
这些结果表明，Antrodia camphorata 的木质栽培子实体和固态培养菌丝体的甲醇提取物具有优异的抗炎活性，因此具有作为水胶体敷料的附加物的巨大潜力。
