Chen C-C, Chyau C-C, Hseu T-H
Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan.
Lett Appl Microbiol. 2007 Apr;44(4):387-92. doi: 10.1111/j.1472-765X.2006.02087.x.
To investigate the active ingredient in fruiting bodies and to produce it with cultured mycelium in Antrodia camphorata (BCRC 35398).
The volatile components from the fruiting bodies, the liquid cultured broth of A. camphorata and Cinnamomum kanehirae wood were separately isolated by steam distillation-solvent extraction and identified by gas chromatography-mass spectrometry. In the fruiting bodies, a COX-2 inhibitor 2,4,5-trimethoxybenzaldehyde (TMBA) was found to be the most abundant constituent, but was totally absent in its cultured broth and its natural host, C. kanehirae wood. On feeding with the acid-digested sawdust of C. kanehirae wood or vanillin to the broth for culture, TMBA was produced in both cultured broths.
The TMBA identified in fruiting bodies was an active ingredient whose functions consisted with the reported experiences of this mushroom. Feeding vanillin to culture broth could produce TMBA containing mycelium product like its fruiting bodies did.
This study found an active ingredient in fruiting bodies of A. camphorata and elucidated this compound derived from digested sawdust of C. kanehirae wood. A feasible method was also developed to produce TMBA containing mycelium by feeding vanillin.
研究樟芝（BCRC 35398）子实体中的活性成分，并利用培养的菌丝体生产该成分。
采用水蒸气蒸馏 - 溶剂萃取法分别分离樟芝子实体、液体培养 broth 以及樟树木材中的挥发性成分，并用气相色谱 - 质谱联用仪进行鉴定。在子实体中，一种环氧化酶 - 2 抑制剂 2,4,5 - 三甲氧基苯甲醛（TMBA）被发现是含量最丰富的成分，但在其培养 broth 及其天然宿主樟树木材中完全不存在。向培养 broth 中添加经酸消化的樟树木材锯末或香草醛进行培养时，两种培养 broth 中均产生了 TMBA。
在子实体中鉴定出的 TMBA 是一种活性成分，其功能与该蘑菇已报道的功效相符。向培养 broth 中添加香草醛能够像子实体一样产生含 TMBA 的菌丝体产物。
本研究在樟芝子实体中发现了一种活性成分，并阐明了该化合物源自樟树木材的消化锯末。还开发了一种通过添加香草醛生产含 TMBA 菌丝体的可行方法。
