Guo Yue, Guo Lin-Na, Zhu Jun-Fei, Tang Chen-Yi, Feng Yun-Zhi, Zhou Hou-De
Department of Metabolism & Endocrinology, National Clinical Research Center for Metabolic Disease, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
Department of Stomatology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
Int J Endocrinol. 2017;2017:1087017. doi: 10.1155/2017/1087017. Epub 2017 Oct 4.
To explore the differences in salivary BPI fold containing family A, member 1 (BPIFA1) concentration among type 2 diabetes mellitus (T2DM) subjects with various severities of chronic periodontitis and to determine whether BPIFA1 in saliva can be used as a potential biomarker of T2DM.
Unstimulated saliva samples were collected from 44 subjects with T2DM and 44 without T2DM (NDM). Additionally, demographic data and general health parameters, including fasting blood glucose (FBG) and body mass index (BMI), were collected. We also detected full-mouth clinical periodontal parameters including probing pocket depth (PPD), clinical attachment level (CAL), bleeding index (BI), and plaque index (PLI). Salivary BPIFA1, tumor necrosis factor- (TNF-), and interleukin-6 (IL-6) concentrations were also detected.
BPIFA1 in saliva was detected at relatively high levels. T2DM subjects had decreased salivary BPIFA1 concentrations ( = 0.031). In T2DM subjects with nonperiodontitis or severe periodontitis, the level of BPIFA1 was significantly lower compared with that of NDM. Salivary TNF- concentration displayed a similar trend to BPIFA1 in the NDM group.
BPIFA1 protein is rich in saliva and might be used as a potential predictive biomarker of T2DM, especially in patients with severe periodontitis and nonperiodontitis. This trial is registered with ChiCTR-ROC-17010310.
探讨不同严重程度慢性牙周炎的2型糖尿病（T2DM）患者唾液中含BPI折叠家族A成员1（BPIFA1）浓度的差异，并确定唾液中的BPIFA1是否可作为T2DM的潜在生物标志物。
收集44例T2DM患者和44例非T2DM（NDM）患者的非刺激性唾液样本。此外，收集人口统计学数据和一般健康参数，包括空腹血糖（FBG）和体重指数（BMI）。我们还检测了全口临床牙周参数，包括探诊深度（PPD）、临床附着水平（CAL）、出血指数（BI）和菌斑指数（PLI）。同时检测唾液中BPIFA1、肿瘤坏死因子-（TNF-）和白细胞介素-6（IL-6）的浓度。
唾液中检测到相对高水平的BPIFA1。T2DM患者唾液中BPIFA1浓度降低（P = 0.031）。在无牙周炎或重度牙周炎的T2DM患者中，BPIFA1水平显著低于NDM患者。在NDM组中，唾液TNF-浓度与BPIFA1呈现相似趋势。
BPIFA1蛋白在唾液中含量丰富，可能作为T2DM的潜在预测生物标志物，尤其是在重度牙周炎和无牙周炎患者中。本试验已在ChiCTR-ROC-17010310注册。
