Department of Prosthodontics and Crown & Bridge, JSS Dental College and Hospital, JSS Academy of Higher Education and Research, Mysore, Karnataka, India.
Department of Periodontics, Sri Hasanamba Dental College and Hospital, Hassan, Karnataka, India.
Indian J Dent Res. 2024 Jan 1;35(1):28-33. doi: 10.4103/ijdr.ijdr_112_23. Epub 2024 May 30.
Periodontitis and type 2 diabetes are chronic inflammatory diseases that increase inflammatory Interleukin-6 (IL-6) levels that induce the production of advanced glycation end products (AGEs) causing receptor activator of nuclear factor-kappa B ligand (RANKL) expression on osteoclasts, contributing to further alveolar bone destruction.
To assess the role and diagnostic potential of salivary IL-6 (SIL-6) in the detection and evaluation of chronic periodontitis (CP) and tooth loss in type 2 diabetes mellitus (T2DM).
This cross-sectional study comprised 240 subjects aged 30-69 years with minimum of 15 natural teeth. Fasting, unstimulated whole saliva was collected, full-mouth intra-oral examination and periodontal evaluation were performed using PCP-UNC 15 probe and glycaemic (HbA1c) levels were analysed by high-performance liquid chromatography (HPLC) method. Subjects were categorised into four groups of 60 participants each: Group 1 (controls); Group 2 (CP); Group 3 (T2DM with CP); Group 4 (T2DM with CP and tooth loss). Salivary IL-6 levels were quantitatively assessed by enzyme-linked immune sorbent assay method.
Average SIL-6 levels were significantly elevated in Group 4 (T2DM with CP and tooth loss) (P = 0.001) and in severe periodontitis (P = 0.001). Karl Pearson Correlation found a significant association between average SIL-6 and average periodontal pocket depth (APPD) (r = 0.180), average clinical attachment loss ≥3 mm (ACAL3) (r = 0.289) and severity of periodontitis (r = 0.3228). The receiver operating characteristic (ROC) curve depicted an overall sensitivity of 53.3%, specificity of 68.6% and accuracy of 60% in the detection and assessment of CP in T2DM with tooth loss.
IL-6 in saliva is a valuable, non-invasive biomarker in the detection and evaluation of CP in T2DM with tooth loss.
牙周炎和 2 型糖尿病是慢性炎症性疾病，会增加炎症性白细胞介素 6（IL-6）水平，从而诱导晚期糖基化终产物（AGEs）的产生，导致破骨细胞上核因子-κB 配体受体激活剂（RANKL）的表达，进一步导致牙槽骨破坏。
评估唾液白细胞介素 6（SIL-6）在检测和评估 2 型糖尿病（T2DM）伴牙周炎（CP）和牙齿缺失中的作用和诊断潜力。
这项横断面研究包括 240 名年龄在 30-69 岁、至少有 15 颗天然牙齿的受试者。采集空腹、非刺激全唾液，使用 PCP-UNC 15 探针进行全口口腔内检查和牙周评估，并通过高效液相色谱（HPLC）法分析血糖（HbA1c）水平。受试者分为 4 组，每组 60 名：第 1 组（对照组）；第 2 组（CP 组）；第 3 组（T2DM 伴 CP 组）；第 4 组（T2DM 伴 CP 和牙齿缺失组）。通过酶联免疫吸附测定法定量评估唾液白细胞介素 6 水平。
第 4 组（T2DM 伴 CP 和牙齿缺失组）的平均 SIL-6 水平显著升高（P=0.001），且在重度牙周炎中也显著升高（P=0.001）。卡尔·皮尔逊相关性分析发现，平均 SIL-6 与平均牙周袋深度（APPD）（r=0.180）、平均临床附着丧失≥3mm（ACAL3）（r=0.289）和牙周炎严重程度（r=0.3228）之间存在显著相关性。受试者工作特征（ROC）曲线显示，在检测和评估 T2DM 伴牙齿缺失的 CP 中，SIL-6 的总体敏感性为 53.3%，特异性为 68.6%，准确性为 60%。
唾液中的白细胞介素 6 是检测和评估 T2DM 伴牙齿缺失的 CP 的一种有价值的、非侵入性的生物标志物。
