Waghmode Samadhan, Dama Laxmikant, Hingamire Tejashri, Bharti Nidhi, Doijad Swapnil, Suryavanshi Mangesh
Department of Microbiology, Elphinstone College, Mumbai 400032.
Department of Zoology, DBF Dayanand College of Arts and Science, Solapur 413002.
J Genomics. 2017 Oct 8;5:124-127. doi: 10.7150/jgen.21724. eCollection 2017.
strain SAMM, a biosurfactant producing moderately halophilic marine bacterium was isolated from Indian Arabian coastline sea water. The strain was found to tolerate up to 2.7 M NaCl indicating osmotic stress sustainable physiological systems. We report here the draft genome sequence of SAMM, as a candidate bacterium for bioactive surfactant producer. The whole genome sequence with 161 scaffolds, 4,414,932 bp and 44.8% of G+C content for SAMM was obtained using Illumina MiSeq sequencing technology. Annotation was added by the PGAP and RAST prokaryotic genome annotation service and shown 4,247 coding sequences, 123 RNAs genes, classified in 453 subsystems. Several genes encoding enzymatic activities against the high molecular weight polysaccharides, osmotic stress response and siderophore synthesis of potential biotechnological importance were identified in the genome.
菌株SAMM是一种产生物表面活性剂的中度嗜盐海洋细菌，从印度阿拉伯海岸线的海水中分离得到。该菌株被发现能够耐受高达2.7 M的NaCl，表明其具有可承受渗透胁迫的生理系统。我们在此报告SAMM的基因组草图序列，它是生物活性表面活性剂产生菌的候选菌株。使用Illumina MiSeq测序技术获得了SAMM的全基因组序列，该序列有161个支架，4,414,932 bp，G+C含量为44.8%。通过PGAP和RAST原核基因组注释服务添加注释，结果显示有4,247个编码序列、123个RNA基因，归类于453个子系统。在基因组中鉴定出了几个编码针对高分子量多糖的酶活性、渗透胁迫反应和具有潜在生物技术重要性的铁载体合成的基因。
