Patel Radhika K, Shah Ravi K, Prajapati Vimal S, Patel Kamlesh C, Trivedi Ujjval B
P. G. Department of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Bakrol, Gujarat, 388315, India.
Texas A&M Agrilife Research and Extension Center, Texas A&M University System, Vernon, TX, USA.
Curr Microbiol. 2021 Apr;78(4):1238-1244. doi: 10.1007/s00284-021-02380-5. Epub 2021 Feb 25.
Acinetobacter indicus strain UBT1 has shown efficient lipase (243 U ml) and biosurfactant (61.1% E24% emulsification and surface tension reduction to 37.7 mN m) production capabilities using agro-industrial waste as sole carbon source. We report here the draft genome sequence of A. indicus strain UBT1 having genome size of 2.97 Mb with 45.90% GC content. Total 2721 coding genes were predicted using National Center for Biotechnology Information-Prokaryotic Genome Annotation Pipeline (NCBI-PGAP). The whole genome shotgun project sequence data are accessible through NCBI Gene Bank under accession no. JABFOI000000000. PGAP annotation revealed the presence of the triacylglycerol lipase, phospholipase etc., that circuitously confers the oil consumption competency to the strain UBT1. Rapid Annotation using the Subsystem Technology (RAST) server used for mapping the genes to the subsystem resulted in 278 subsystem with 30% subsystem coverage. The draft genome data can be used to exploit the A. indicus strain UBT1 for its advance biotechnological application and also for further comparative genomic studies.
印度不动杆菌菌株UBT1已显示出利用农业工业废料作为唯一碳源高效生产脂肪酶（243 U/ml）和生物表面活性剂（E24乳化率61.1%，表面张力降低至37.7 mN/m）的能力。我们在此报告印度不动杆菌菌株UBT1的基因组草图序列，其基因组大小为2.97 Mb，GC含量为45.90%。使用美国国立生物技术信息中心原核生物基因组注释管道（NCBI-PGAP）预测了总共2721个编码基因。全基因组鸟枪法测序项目序列数据可通过NCBI基因库获取，登录号为JABFOI000000000。PGAP注释显示存在三酰甘油脂肪酶、磷脂酶等，这些间接赋予了菌株UBT1消耗油脂的能力。使用子系统技术快速注释（RAST）服务器将基因映射到子系统，结果得到278个子系统，子系统覆盖率为30%。基因组草图数据可用于开发印度不动杆菌菌株UBT1以进行先进的生物技术应用，也可用于进一步的比较基因组研究。
