Lodz University of Technology, Institute of Fermentation Technology and Microbiology, Wolczanska 171/173, 90-024, Lodz, Poland.
Lodz University of Technology, Institute of Food Technology and Analysis, Stefanowskiego 4/10, 90-024, Lodz, Poland.
Probiotics Antimicrob Proteins. 2018 Jun;10(2):186-200. doi: 10.1007/s12602-017-9344-0.
The antifungal activity of Lactobacillus pentosus ŁOCK 0979 depends both on the culture medium and on the fungal species. In the control medium, the strain exhibited limited antagonistic activity against indicator food-borne molds and yeasts. However, the supplementation of the bacterial culture medium with polyols (erythritol, lactitol, maltitol, mannitol, sorbitol, xylitol) or their galactosyl derivatives (gal-erythritol, gal-sorbitol, gal-xylitol) enhanced the antifungal properties of Lactobacillus pentosus ŁOCK 0979. Its metabolites were identified and quantified by enzymatic methods, HPLC, UHPLC-MS coupled with QuEChERS, and GC-MS. The presence of polyols and gal-polyols significantly affected the acid metabolite profile of the bacterial culture supernatant. In addition, lactitol and mannitol were used by bacteria as alternative carbon sources. A number of compounds with potential antifungal properties were identified, such as phenyllactic acid, hydroxyphenyllactic acid, and benzoic acid. Lactobacillus bacteria cultivated with mannitol synthesized hydroxy-fatty acids, including 2-hydroxy-4-methylpentanoic acid, a well-described antifungal agent. Scanning electron microscopy (SEM) and light microscopy confirmed a strong antifungal effect of L. pentosus ŁOCK 0979.
植物乳杆菌 ŁOCK 0979 的抗真菌活性既取决于培养基，也取决于真菌种类。在对照培养基中，该菌株对指示性食源霉菌和酵母的拮抗活性有限。然而，在细菌培养基中添加多元醇（赤藓糖醇、乳糖醇、麦芽糖醇、甘露醇、山梨糖醇、木糖醇）或其半乳糖基衍生物（半乳糖赤藓糖醇、半乳糖山梨糖醇、半乳糖木糖醇）增强了植物乳杆菌 ŁOCK 0979 的抗真菌特性。通过酶法、HPLC、UHPLC-MS 与 QuEChERS 联用和 GC-MS 对其代谢物进行了鉴定和定量。多元醇和半乳糖多元醇的存在显著影响了细菌培养液上清液的酸代谢物图谱。此外，乳糖醇和甘露醇被细菌用作替代碳源。鉴定出了一些具有潜在抗真菌特性的化合物，如苯乳酸、羟基苯乳酸和苯甲酸。培养在甘露醇中的乳杆菌合成了羟基脂肪酸，包括 2-羟基-4-甲基戊酸，这是一种公认的抗真菌剂。扫描电子显微镜 (SEM) 和明场显微镜证实了植物乳杆菌 ŁOCK 0979 的强烈抗真菌作用。
