New York University, New York, USA.
University College London, London, UK.
J Physiol. 2018 Jan 15;596(2):145-162. doi: 10.1113/JP273463. Epub 2017 Dec 4.
An important consideration when probing the function of any neuron is to uncover the source of synaptic input onto the cell, its intrinsic physiology and efferent targets. Over the years, electrophysiological approaches have generated considerable insight into these properties in a variety of cortical neuronal subtypes and circuits. However, as researchers explore neuronal function in greater detail, they are increasingly turning to optical techniques to bridge the gap between local network interactions and behaviour. The application of optical methods has increased dramatically over the past decade, spurred on by the optogenetic revolution. In this review, we provide an account of recent innovations, providing researchers with a primer detailing circuit mapping strategies in the cerebral cortex. We will focus on technical aspects of performing neurotransmitter uncaging and channelrhodopsin-assisted circuit mapping, with the aim of identifying common pitfalls that can negatively influence the collection of reliable data.
当探究任何神经元的功能时，一个重要的考虑因素是揭示细胞上突触输入的来源、其内在生理学和传出靶标。多年来，电生理学方法已经在各种皮质神经元亚型和回路中对这些特性产生了相当大的深入了解。然而，随着研究人员更详细地探索神经元功能，他们越来越多地转向光学技术来弥合局部网络相互作用和行为之间的差距。在过去的十年中，光学方法的应用急剧增加，这得益于光遗传学革命。在这篇综述中，我们提供了最近创新的描述，为研究人员提供了详细的大脑皮层电路映射策略入门。我们将重点介绍进行神经递质光解笼和通道视紫红质辅助电路映射的技术方面，目的是确定可能会对收集可靠数据产生负面影响的常见陷阱。
