Department of Veterinary Internal Medicine, College of Veterinary Medicine, Chonnam National University, Gwangju, South Korea.
Asan Institute for Life Sciences, College of Medicine, University of Ulsan, Seoul, South Korea.
J Dermatol Sci. 2018 Jan;89(1):27-32. doi: 10.1016/j.jdermsci.2017.10.006. Epub 2017 Oct 18.
Atopic dermatitis (AD) is recently increasing among populations, but the underlying mechanisms remain controversial. Interactions between the gut microbiota and mucosal immunity are considered to be a crucial etiology. Fructooligosaccharide (FOS), prebiotics have been reported as activators of the gut microbiota.
The aim of this study was to investigate the effects of kestose, the smallest FOS and FOS on atopic dermatitis in mice.
An AD mouse model was developed by (ovalbumin) epidermal sensitization using BALB/c mice. Kestose (1%, 5%, and 10%) or FOS (5%, positive control) was orally administered throughout the study.
In comparison with the values observed for the control AD mice, transepidermal water loss (TEWL), clinical score, and skin inflammation on histopathology were significantly decreased by the oral administration of kestose. Total IgE, thymic stromal lymphopoietin (TSLP) in skin, and IL-4 were also suppressed by this administration. In addition, the population of CD4Foxp3 cells in mesenteric lymph nodes (MLNs) and acetate concentrations in feces were significantly increased by kestose treatment.
These findings suggest that kestose activates the gut immune system to induce the tolerance against allergic skin inflammations in AD.
特应性皮炎（AD）在人群中的发病率最近呈上升趋势，但潜在机制仍存在争议。肠道微生物群与黏膜免疫之间的相互作用被认为是一个关键的病因。低聚果糖（FOS）作为肠道微生物群的激活剂已被报道。
本研究旨在研究蔗果三糖，最小的 FOS 和 FOS 对小鼠特应性皮炎的影响。
采用 BALB/c 小鼠进行（卵清蛋白）表皮致敏建立 AD 小鼠模型。在整个研究过程中，通过口服给予蔗果三糖（1%、5%和 10%）或 FOS（阳性对照，5%）。
与对照 AD 小鼠相比，口服蔗果三糖可显著降低经表皮水分流失（TEWL）、临床评分和皮肤组织病理学炎症。这种给药还抑制了总 IgE、皮肤中的胸腺基质淋巴细胞生成素（TSLP）和 IL-4。此外，口服蔗果三糖还可显著增加肠系膜淋巴结（MLN）中 CD4Foxp3 细胞的数量和粪便中乙酸盐的浓度。
这些发现表明，蔗果三糖激活肠道免疫系统，诱导 AD 中对过敏性皮肤炎症的耐受。
