Department of Animal Resources Technology, Gyeongnam National University of Science and Technology, Jinju 52725, Republic of Korea.
Department of Animal Science, Chonnam National University, Gwangju 61186, Republic of Korea 3 Department of Animal Science & Biotechnology, Gyeongnam National University of Science and Technology, Jinju 52725, Republic of Korea.
J Microbiol Biotechnol. 2020 Sep 28;30(9):1343-1354. doi: 10.4014/jmb.2005.05017.
Atopic dermatitis (AD) is a skin disorder that causes chronic itch. We investigated the inhibitory effects of a mixture of prebiotic short-chain galacto-oligosaccharides and long-chain fructooligosaccharides (scGOS/lcFOS), inulin, or β-glucan on AD development in 1-chloro-2,4- dinitrobenzene (DNCB)-treated NC/Nga mice. Mice were randomly assigned to six groups: untreated mice, AD control, positive control (DNCB-treated NC/Nga mice fed a dietary supplement of Zyrtec), and DNCB-treated NC/Nga mice fed a dietary supplement of prebiotics such as scGOS/lcFOS (T1), inulin (T2), or β-glucan (T3). The prebiotic treatment groups (T1, T2, and T3) showed suppression of AD symptoms, Th2 cell differentiation, and AD-like skin lesions induced by DNCB. In addition, prebiotic treatment also reduced the number of microorganisms such as , which is associated with AD symptoms, and increased the levels of and , which are associated with alleviation of AD symptoms. Our findings demonstrate the inhibitory effects of prebiotics on AD development by improving the Th1/Th2 cytokine balance and beneficial symbiotic microorganisms in in vitro and in vivo models.
特应性皮炎（AD）是一种引起慢性瘙痒的皮肤疾病。我们研究了短链半乳糖寡糖和长链菊粉混合物、菊糖或β-葡聚糖对 1-氯-2,4-二硝基苯（DNCB）处理的 NC/Nga 小鼠 AD 发展的抑制作用。将小鼠随机分为六组：未处理的小鼠、AD 对照组、阳性对照（DNCB 处理的 NC/Nga 小鼠用 Zyrtec 进行饮食补充）和 DNCB 处理的 NC/Nga 小鼠用 scGOS/lcFOS（T1）、菊糖（T2）或β-葡聚糖（T3）进行饮食补充。结果显示，益生元治疗组（T1、T2 和 T3）抑制了 DNCB 诱导的 AD 症状、Th2 细胞分化和 AD 样皮肤损伤。此外，益生元治疗还减少了与 AD 症状相关的微生物如 的数量，并增加了与 AD 症状缓解相关的 和 的水平。我们的研究结果表明，益生元通过改善体外和体内模型中的 Th1/Th2 细胞因子平衡和有益共生微生物，对 AD 的发展具有抑制作用。
