Laboratory of Microbiology, Research Laboratory for Microorganisms and Human Disease, Faculty of Medicine, Habib Bourguiba University Hospital, Sfax, Tunisia.
Int J Infect Dis. 2017 Dec;65:110-115. doi: 10.1016/j.ijid.2017.10.015. Epub 2017 Oct 27.
To analyze the serotype distribution of Streptococcus pneumoniae clinical isolates collected in the south of Tunisia over a 5-year period in different age groups and to assess their antimicrobial susceptibility patterns.
A total of 305 non-duplicate S. pneumoniae isolates were collected between January 2012 and December 2016 at the university hospital in Sfax, Tunisia. All isolates were serotyped by multiplex PCR. The antibiotic susceptibility of all isolates was determined using the disk diffusion test or Etest assay.
Among the 305 pneumococcal isolates, 76 (24.9%) were invasive and 229 (75.1%) were non-invasive. The most common serotypes were 19F (20%), 14 (16.7%), 3 (9.2%), 23F (7.5%), 19A (5.9%), and 6B (5.9%). Potential immunization coverage rates for pneumococcal conjugate vaccines PCV7, PCV10, and PCV13 were 58%, 59.3%, and 78.7%, respectively. Three-quarters (75.3%) of pneumococcal isolates were non-susceptible to penicillin. The resistance rate to erythromycin was 71.4%. Only two isolates were resistant to levofloxacin.
19F and 14 were the most prevalent serotypes in the south of Tunisia. The inclusion of a PCV in the immunization program could be useful for reducing the burden of pneumococcal diseases. The high resistance rate to penicillin and macrolides is alarming. Prudent use of antibiotics is crucial to prevent the selection of multidrug-resistant pneumococci.
分析在突尼斯南部地区，5 年间不同年龄组采集的肺炎链球菌临床分离株的血清型分布，并评估其抗菌药物敏感性模式。
2012 年 1 月至 2016 年 12 月期间，在突尼斯苏塞大学医院共收集了 305 株非重复的肺炎链球菌分离株。所有分离株均采用多重 PCR 进行血清型分型。采用纸片扩散法或 Etest 法检测所有分离株的抗生素敏感性。
在 305 株肺炎链球菌分离株中，76 株（24.9%）为侵袭性，229 株（75.1%）为非侵袭性。最常见的血清型为 19F（20%）、14（16.7%）、3（9.2%）、23F（7.5%）、19A（5.9%）和 6B（5.9%）。肺炎球菌结合疫苗 PCV7、PCV10 和 PCV13 的潜在免疫覆盖率分别为 58%、59.3%和 78.7%。四分之三（75.3%）的肺炎链球菌分离株对青霉素不敏感。对红霉素的耐药率为 71.4%。仅有 2 株分离株对左氧氟沙星耐药。
19F 和 14 是突尼斯南部最常见的血清型。在免疫规划中纳入 PCV 可能有助于减轻肺炎球菌疾病的负担。青霉素和大环内酯类药物的高耐药率令人担忧。谨慎使用抗生素对于防止选择多药耐药肺炎球菌至关重要。
