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截叶蒲桃叶绿体基因组的全序列测定与比较分析

Complete sequence and comparative analysis of the chloroplast genome of Plinia trunciflora.

作者信息

Eguiluz Maria, Yuyama Priscila Mary, Guzman Frank, Rodrigues Nureyev Ferreira, Margis Rogerio

机构信息

Programa de Pós-Graduação em Genética e Biologia Molecular, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.

Departamento de Biofísica, Centro de Biotecnologia, Laboratório de Genomas e Populações de Plantas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.

出版信息

Genet Mol Biol. 2017 Oct-Dec;40(4):871-876. doi: 10.1590/1678-4685-GMB-2017-0096. Epub 2017 Nov 6.

Abstract

Plinia trunciflora is a Brazilian native fruit tree from the Myrtaceae family, also known as jaboticaba. This species has great potential by its fruit production. Due to the high content of essential oils in their leaves and of anthocyanins in the fruits, there is also an increasing interest by the pharmaceutical industry. Nevertheless, there are few studies focusing on its molecular biology and genetic characterization. We herein report the complete chloroplast (cp) genome of P. trunciflora using high-throughput sequencing and compare it to other previously sequenced Myrtaceae genomes. The cp genome of P. trunciflora is 159,512 bp in size, comprising inverted repeats of 26,414 bp and single-copy regions of 88,097 bp (LSC) and 18,587 bp (SSC). The genome contains 111 single-copy genes (77 protein-coding, 30 tRNA and four rRNA genes). Phylogenetic analysis using 57 cp protein-coding genes demonstrated that P. trunciflora, Eugenia uniflora and Acca sellowiana form a cluster with closer relationship to Syzygium cumini than with Eucalyptus. The complete cp sequence reported here can be used in evolutionary and population genetics studies, contributing to resolve the complex taxonomy of this species and fill the gap in genetic characterization.

摘要

截叶嘉宝果是一种原产于巴西的桃金娘科果树,也被称为嘉宝果。该物种因其果实产量具有巨大潜力。由于其叶片中精油含量高,果实中花青素含量高,制药行业对其兴趣也在日益增加。然而,很少有研究关注其分子生物学和遗传特征。我们在此报告利用高通量测序获得的截叶嘉宝果完整叶绿体(cp)基因组,并将其与其他先前测序的桃金娘科基因组进行比较。截叶嘉宝果的cp基因组大小为159,512 bp,包括26,414 bp的反向重复序列以及88,097 bp(LSC)和18,587 bp(SSC)的单拷贝区域。该基因组包含111个单拷贝基因(77个蛋白质编码基因、30个tRNA基因和4个rRNA基因)。使用57个cp蛋白质编码基因进行的系统发育分析表明,截叶嘉宝果、巴西番樱桃和费约果形成一个聚类,与乌墨的关系比与桉树更近。此处报道的完整cp序列可用于进化和群体遗传学研究,有助于解决该物种复杂的分类问题并填补遗传特征方面的空白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fb/5738614/cf3c9d30c545/1415-4757-gmb-1678-4685-GMB-2017-0096-gf01.jpg

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